Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Purification
This antibody is purified through a protein A column, followed by peptide affinity purification.
Immunogen
This cGKII antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 714-744 amino acids from the C-terminal region of human cGKII.
PRKG2
Reactivity: Human
ELISA, IHC
Host: Rabbit
Polyclonal
unconjugated
Application Notes
WB: 1:1000. IHC-P: 1:50~100. IHC-P: 1:50~100
Restrictions
For Research Use only
Format
Liquid
Buffer
Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C,-20 °C
Storage Comment
Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date
6 months
Zhu, Yao, Wu, Qian, Wu, Chen: "Type II cGMP-dependent protein kinase directly inhibits HER2 activation of gastric cancer cells." in: Molecular medicine reports, Vol. 13, Issue 2, pp. 1909-13, (2016) (PubMed).
Yao, Wu, Zhu, Qian, Chen et al.: "Nitric oxide/cyclic guanosine monophosphate inducers sodium nitroprusside and L-arginine inhibit the proliferation of gastric cancer cells via the activation of type II cyclic guanosine ..." in: Oncology letters, Vol. 10, Issue 1, pp. 479-484, (2015) (PubMed).
Jiang, Wu, Wu, Lan, Wang, Qian, Chen: "Type II cyclic guanosine monophosphate-dependent protein kinase inhibits epidermal growth factor receptor activation in different cancer cell lines." in: Oncology letters, Vol. 9, Issue 6, pp. 2781-2786, (2015) (PubMed).
Rangaswami, Marathe, Zhuang, Chen, Yeh, Frangos, Boss, Pilz: "Type II cGMP-dependent protein kinase mediates osteoblast mechanotransduction." in: The Journal of biological chemistry, Vol. 284, Issue 22, pp. 14796-808, (2009) (PubMed).
Target
PRKG2
(Protein Kinase, CGMP-Dependent, Type II (PRKG2))
MGC82580 antibody, PRKG2 antibody, si:dkey-18e17.2 antibody, PRKGR2 antibody, cGKII antibody, GDPKII antibody, AW212535 antibody, CGKII antibody, Prkgr2 antibody, cGK-II antibody, protein kinase, cGMP-dependent, type II antibody, protein kinase, cGMP-dependent, type II L homeolog antibody, cGMP-dependent protein kinase 2 antibody, PRKG2 antibody, prkg2.L antibody, prkg2 antibody, LOC100541294 antibody, Prkg2 antibody
Background
CGKII is thought to play a key role in a diverse set of physiological pathway. cGKII may mediate intestinal secretion of water and electrolytes induced by the E. coli toxin STa and the intestinal peptide guanylin. Edentification of the pathway that mediates intestinal fluid secretion by E. coli STa has potential medical implications because STa causes traveler's diarrhea and about 50 % of infant mortality in developing countries. Transfection experiments in human cells disclose that cGKII phosphorylates SOX9 and attenuates SOX9 function by inhibiting its nuclear entry. Impaired differentiation of cultured KMI chondrocytes can be restored by silencing Sox9 by RNA interference. cGKII is postulated to be a molecular switch that couples the cessation of proliferation and the start of hypertrophic chondrocyte differentiation through attenuating SOX9 function.