Caspase 3 antibody (AA 60-90)

Catalog No. ABIN391813

Product Details anti-Caspase 3 Antibody

Target: Caspase 3 (CASP3)

Binding Specificity: AA 60-90

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Caspase 3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS)

Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogen: This Caspase-3 (CASP3) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 60-90 amino acids from the Central region of human Caspase-3 (CASP3).

Clone: RB14696

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:2000. WB: 1:1000. WB: 1:1000. IHC-P: 1:10~50. FC: 1:10~50

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

References for anti-Caspase 3 antibody (ABIN391813)

Zhao, Xia, Zhang, Xiong, Li, Yan, Chen, Wang, Wang, Gao, Lee, Li, Wang, Zhang, Tao: "Nucleostemin dysregulation contributes to ischemic vulnerability of diabetic hearts: Role of ribosomal biogenesis." in: Journal of molecular and cellular cardiology, Vol. 108, pp. 106-113, (2018) (PubMed).

Ling, Song, Fan, Feng, Liu, Yang, Wang, Li, Tian, Zhao, Liu, Huang, Hou, Xu, Shi, Li: "Combination of metformin and sorafenib suppresses proliferation and induces autophagy of hepatocellular carcinoma via targeting the mTOR pathway." in: International journal of oncology, Vol. 50, Issue 1, pp. 297-309, (2017) (PubMed).

Guo, Shao, Chen, Xu, Zhang, Liu: "Expression of pituitary tumor-transforming 2 in human glioblastoma cell lines and its role in glioblastoma tumorigenesis." in: Experimental and therapeutic medicine, Vol. 11, Issue 5, pp. 1847-1852, (2016) (PubMed).

Liu, Xie, Wang, Liu, Liu, Sun, Niu: "Ubenimex inhibits cell proliferation, migration and invasion in renal cell carcinoma: the effect is autophagy-associated." in: Oncology reports, Vol. 33, Issue 3, pp. 1372-80, (2015) (PubMed).

Tai, Sun, Liu, Ding, Hsia, Bhuta, Thor, Damoiseaux, Liang, Huang: "Combination of Rad001 (everolimus) and propachlor synergistically induces apoptosis through enhanced autophagy in prostate cancer cells." in: Molecular cancer therapeutics, Vol. 11, Issue 6, pp. 1320-31, (2012) (PubMed).

Target Details for Caspase 3

Target: Caspase 3 (CASP3)

Alternative Name: Caspase-3 (CASP3) (CASP3 Products)

Synonyms: CPP32 antibody, CPP32B antibody, SCA-1 antibody, A830040C14Rik antibody, AC-3 antibody, Apopain antibody, CC3 antibody, Caspase-3 antibody, Lice antibody, Yama antibody, mldy antibody, xcpp32 antibody, casp3 antibody, zgc:100890 antibody, CASP-3 antibody, caspase-3 antibody, caspase 3 antibody, caspase 3 S homeolog antibody, caspase 3, apoptosis-related cysteine peptidase a antibody, caspase 3, apoptosis-related cysteine peptidase antibody, CASP3 antibody, Casp3 antibody, casp3.S antibody, casp3a antibody

Background: CASP3 is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease.

Molecular Weight: 31608

Gene ID: 836

NCBI Accession: NP_004337, NP_116786

UniProt: P42574

Pathways: Apoptosis, Caspase Cascade in Apoptosis, Sensory Perception of Sound, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Activated T Cell Proliferation