HIPK3 antibody (C-Term)

Catalog No. ABIN391779

Product Details anti-HIPK3 Antibody

Target: HIPK3 (Homeodomain Interacting Protein Kinase 3 (HIPK3))

Binding Specificity: AA 1127-1156, C-Term

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HIPK3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Predicted Reactivity: M, Rat

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This HIPK3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1127-1156 amino acids from the C-terminal region of human HIPK3.

Clone: RB3241

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:2000. WB: 1:1000. IHC-P: 1:25

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

References for anti-HIPK3 antibody (ABIN391779)

Lan, Wu, Shih, Chung: "Death-associated protein 6 (Daxx) mediates cAMP-dependent stimulation of Cyp11a1 (P450scc) transcription." in: The Journal of biological chemistry, Vol. 287, Issue 8, pp. 5910-6, (2012) (PubMed).

Tiefenbach, Moll, Nelson, Hu, Baev, Kislinger, Krause: "A live zebrafish-based screening system for human nuclear receptor ligand and cofactor discovery." in: PLoS ONE, Vol. 5, Issue 3, pp. e9797, (2010) (PubMed).

Lan, Li, Lin, Lai, Chung: "Cyclic AMP stimulates SF-1-dependent CYP11A1 expression through homeodomain-interacting protein kinase 3-mediated Jun N-terminal kinase and c-Jun phosphorylation." in: Molecular and cellular biology, Vol. 27, Issue 6, pp. 2027-36, (2007) (PubMed).

Target Details for HIPK3

Target: HIPK3 (Homeodomain Interacting Protein Kinase 3 (HIPK3))

Alternative Name: HIPK3 (HIPK3 Products)

Background: HIPK3 negatively regulates apoptosis by promoting FADD phosphorylation. This kinase enhances androgen receptor-mediated transcription, and may act as a transcriptional corepressor for NK homeodomain transcription factors.

Molecular Weight: 133743

Gene ID: 10114

NCBI Accession: NP_001041665, NP_001265091, NP_001265092, NP_005725

UniProt: Q9H422