Ketohexokinase antibody (C-Term)

Catalog No. ABIN391089

Product Details anti-Ketohexokinase Antibody

Target: Ketohexokinase (KHK)

Binding Specificity: AA 251-281, C-Term

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Ketohexokinase antibody is un-conjugated

Application: Western Blotting (WB)

Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogen: This Ketohexokinase (KHK) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 251-281 amino acids from the C-terminal region of human Ketohexokinase (KHK).

Clone: RB05412

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:1000. WB: 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

References for anti-Ketohexokinase antibody (ABIN391089)

Springer, Lindbloom-Hawley, Schermerhorn: "Tissue expression of ketohexokinase in cats." in: Research in veterinary science, Vol. 87, Issue 1, pp. 115-7, (2009) (PubMed).

Diggle, Shires, Leitch, Brooke, Carr, Markham, Hayward, Asipu, Bonthron: "Ketohexokinase: expression and localization of the principal fructose-metabolizing enzyme." in: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 57, Issue 8, pp. 763-74, (2009) (PubMed).

Target Details for Ketohexokinase

Target: Ketohexokinase (KHK)

Abstract: KHK Products

Background: Ketohexokinase (KHK), or fructokinase, catalyzes conversion of fructose to fructose-1-phosphate. Splice variant 1 is the highly active form found in liver, renal cortex, and small intestine, while splice variant 2 is the lower activity form found in most other tissues. KHK, like glucokinase (GCK) and glucokinase regulator (GCKR), is present in both liver and pancreatic islets. The inhibition of GCK by GCKR is blocked by binding of fructose-1-phosphate to GCKR. The chromosomal proximity of the metabolically connected GCKR and KHK genes has a genetic linkage in type 2 diabetes. Fructosuria, or hepatic fructokinase deficiency, is a benign, asymptomatic defect of intermediary metabolism associated with heterozygosity for G50R and A43T mutations in KHK.

Molecular Weight: 32523

Gene ID: 3795

NCBI Accession: NP_000212, NP_006479

UniProt: P50053