PDK4 antibody (C-Term)

Catalog No. ABIN391039

Product Details anti-PDK4 Antibody

Target: PDK4 (Pyruvate Dehydrogenase Kinase, Isozyme 4 (PDK4))

Binding Specificity: AA 382-410, C-Term

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PDK4 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This PDK4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 382-410 amino acids from the C-terminal region of human PDK4.

Clone: RB03041

Isotype: Ig Fraction

Application Details

Application Notes: IF: 1:100. WB: 1:2000. WB: 1:2000. WB: 1:2000. IHC-P: 1:10~50. IHC-P: 1:25

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots.

Expiry Date: 6 months

References for anti-PDK4 antibody (ABIN391039)

Cole, Abd Jamil, Heather, Murray, Sutton, Slingo, Sebag-Montefiore, Tan, Aksentijević, Gildea, Stuckey, Yeoh, Carr, Evans, Aasum, Schofield, Ratcliffe, Neubauer, Robbins, Clarke: "On the pivotal role of PPARα in adaptation of the heart to hypoxia and why fat in the diet increases hypoxic injury." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 30, Issue 8, pp. 2684-97, (2017) (PubMed).

Zhang, Wu, Choiniere, Yang, Huang, Bennett, Wang: "Arsenic silences hepatic PDK4 expression through activation of histone H3K9 methylatransferase G9a." in: Toxicology and applied pharmacology, Vol. 304, pp. 42-7, (2017) (PubMed).

Zhang, Zhang, Geng, Yi, Huo, Talmon, Kim, Wang, Wang: "Transforming Growth Factor β Mediates Drug Resistance by Regulating the Expression of Pyruvate Dehydrogenase Kinase 4 in Colorectal Cancer." in: The Journal of biological chemistry, Vol. 291, Issue 33, pp. 17405-16, (2017) (PubMed).

Li, Li, Hu, Lei, Xiong, Ji, Yin, Wei, Hu: "The microRNA-182-PDK4 axis regulates lung tumorigenesis by modulating pyruvate dehydrogenase and lipogenesis." in: Oncogene, Vol. 36, Issue 7, pp. 989-998, (2017) (PubMed).

Jiang, de Castro Barbosa, Massart, Deshmukh, Löfgren, Duque-Guimaraes, Ozilgen, Osler, Chibalin, Zierath: "Diacylglycerol kinase-δ regulates AMPK signaling, lipid metabolism, and skeletal muscle energetics." in: American journal of physiology. Endocrinology and metabolism, Vol. 310, Issue 1, pp. E51-60, (2016) (PubMed).

Mazar, Qi, Lee, Marchica, Govindarajan, Shelley, Li, Ray, Perera: "MicroRNA 211 Functions as a Metabolic Switch in Human Melanoma Cells." in: Molecular and cellular biology, Vol. 36, Issue 7, pp. 1090-108, (2016) (PubMed).

Walter, Thomalla, Bruhn, Fagan, Zehowski, Yee, Skildum: "Altered regulation of PDK4 expression promotes antiestrogen resistance in human breast cancer cells." in: SpringerPlus, Vol. 4, pp. 689, (2015) (PubMed).

Kamarajugadda, Stemboroski, Cai, Simpson, Nayak, Tan, Lu: "Glucose oxidation modulates anoikis and tumor metastasis." in: Molecular and cellular biology, Vol. 32, Issue 10, pp. 1893-907, (2012) (PubMed).

Haldar, Jeyaraj, Anand, Zhu, Lu, Prosdocimo, Eapen, Kawanami, Okutsu, Brotto, Fujioka, Kerner, Rosca, McGuinness, Snow, Russell, Gerber, Bai, Yan, Nosek, Brotto, Hoppel, Jain: "Kruppel-like factor 15 regulates skeletal muscle lipid flux and exercise adaptation." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 109, Issue 17, pp. 6739-44, (2012) (PubMed).

Williams, Wan, Frier, Bell, Field, Wright: "Dietary supplementation with vitamin E and C attenuates dexamethasone-induced glucose intolerance in rats." in: American journal of physiology. Regulatory, integrative and comparative physiology, Vol. 302, Issue 1, pp. R49-58, (2011) (PubMed).

Long, Kostovski, Boon, Hjeltnes, Krook, Widegren: "Differential expression of metabolic genes essential for glucose and lipid metabolism in skeletal muscle from spinal cord injured subjects." in: Journal of applied physiology (Bethesda, Md. : 1985), Vol. 110, Issue 5, pp. 1204-10, (2011) (PubMed).

Phillips, Aponte, Covian, Balaban: "Intrinsic protein kinase activity in mitochondrial oxidative phosphorylation complexes." in: Biochemistry, Vol. 50, Issue 13, pp. 2515-29, (2011) (PubMed).

Dunford, Herbst, Jeoung, Gittings, Inglis, Vandenboom, LeBlanc, Harris, Peters: "PDH activation during in vitro muscle contractions in PDH kinase 2 knockout mice: effect of PDH kinase 1 compensation." in: American journal of physiology. Regulatory, integrative and comparative physiology, Vol. 300, Issue 6, pp. R1487-93, (2011) (PubMed).

Attia, Connnaughton, Boone, Wang, Elam, Ness, Cook, Park: "Regulation of pyruvate dehydrogenase kinase 4 (PDK4) by thyroid hormone: role of the peroxisome proliferator-activated receptor gamma coactivator (PGC-1 alpha)." in: The Journal of biological chemistry, Vol. 285, Issue 4, pp. 2375-85, (2010) (PubMed).

Dateki, Kunitomo, Yoshioka, Yanai, Nakasono, Negishi: "Adaptive gene regulation of pyruvate dehydrogenase kinase isoenzyme 4 in hepatotoxic chemical-induced liver injury and its stimulatory potential for DNA repair and cell proliferation." in: Journal of receptor and signal transduction research, Vol. 31, Issue 1, pp. 85-95, (2010) (PubMed).

Zhao, Okuyama, Hashimoto, Tagawa, Jomori, Yang: "Bezafibrate induces myotoxicity in human rhabdomyosarcoma cells via peroxisome proliferator-activated receptor alpha signaling." in: Toxicology in vitro : an international journal published in association with BIBRA, Vol. 24, Issue 1, pp. 154-9, (2010) (PubMed).

Basu, Oudit, Wang, Zhang, Ussher, Lopaschuk, Kassiri: "Type 1 diabetic cardiomyopathy in the Akita (Ins2WT/C96Y) mouse model is characterized by lipotoxicity and diastolic dysfunction with preserved systolic function." in: American journal of physiology. Heart and circulatory physiology, Vol. 297, Issue 6, pp. H2096-108, (2009) (PubMed).

Hsieh, Das, Sambandam, Zhang, Nahlé: "Regulation of the PDK4 isozyme by the Rb-E2F1 complex." in: The Journal of biological chemistry, Vol. 283, Issue 41, pp. 27410-7, (2008) (PubMed).

Nahlé, Hsieh, Pietka, Coburn, Grimaldi, Zhang, Das, Abumrad: "CD36-dependent regulation of muscle FoxO1 and PDK4 in the PPAR delta/beta-mediated adaptation to metabolic stress." in: The Journal of biological chemistry, Vol. 283, Issue 21, pp. 14317-26, (2008) (PubMed).

Long, Glund, Garcia-Roves, Zierath: "Calcineurin regulates skeletal muscle metabolism via coordinated changes in gene expression." in: The Journal of biological chemistry, Vol. 282, Issue 3, pp. 1607-14, (2007) (PubMed).

Target Details for PDK4

Target: PDK4 (Pyruvate Dehydrogenase Kinase, Isozyme 4 (PDK4))

Alternative Name: PDK4 (PDK4 Products)

Synonyms: AV005916 antibody, pyruvate dehydrogenase kinase 4 antibody, pyruvate dehydrogenase kinase, isoenzyme 4 antibody, pdk4 antibody, PDK4 antibody, Pdk4 antibody

Background: PDK4 inhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism.

Molecular Weight: 46469

Gene ID: 5166

NCBI Accession: NP_002603

UniProt: Q16654

Pathways: PI3K-Akt Signaling, Hedgehog Signaling, Carbohydrate Homeostasis, Regulation of Carbohydrate Metabolic Process, Warburg Effect