PKC iota antibody (N-Term)

Catalog No. ABIN391010

Product Details anti-PKC iota Antibody

Target: PKC iota (PRKCI) (Protein Kinase C, iota (PRKCI))

Binding Specificity: AA 176-207, N-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PKC iota antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogen: This PKC iota antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 176-207 amino acids from the N-terminal region of human PKC iota.

Clone: RB01026

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:1000. WB: 1:1000. IHC-P: 1:50~100

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

References for anti-PKC iota antibody (ABIN391010)

Lechuga, Baranwal, Ivanov: "Actin-interacting protein 1 controls assembly and permeability of intestinal epithelial apical junctions." in: American journal of physiology. Gastrointestinal and liver physiology, Vol. 308, Issue 9, pp. G745-56, (2015) (PubMed).

Wang, Li, Du, Lu, Zou: "Significance and expression of atypical protein kinase C-iota in human hepatocellular carcinoma." in: The Journal of surgical research, Vol. 154, Issue 1, pp. 143-9, (2009) (PubMed).

Li, Wang, Liu, Xiao, Lu, Zou: "Correlation of aPKC-iota and E-cadherin expression with invasion and prognosis of cholangiocarcinoma." in: Hepatobiliary & pancreatic diseases international : HBPD INT, Vol. 7, Issue 1, pp. 70-5, (2008) (PubMed).

Target Details for PKC iota

Target: PKC iota (PRKCI) (Protein Kinase C, iota (PRKCI))

Alternative Name: PKC iota (PRKCI Products)

Synonyms: DXS1179E antibody, PKCI antibody, nPKC-iota antibody, aPKC[l] antibody, hal antibody, has antibody, PRKCI antibody, PKC antibody, 2310021H13Rik antibody, AI427505 antibody, PKClambda antibody, Pkci antibody, Pkcl antibody, Prkcl antibody, aPKClambda antibody, mKIAA4165 antibody, protein kinase C iota antibody, protein kinase C, iota antibody, PRKCI antibody, prkci antibody, Prkci antibody

Background: PKC iota belongs to the protein kinase C (PKC) family of serine/threonine protein kinases. The PKC family comprises at least eight members, which are differentially expressed and are involved in a wide variety of cellular processes. This protein kinase is calcium-independent and phospholipid-dependent. It is not activated by phorbolesters or diacylglycerol. This kinase can be recruited to vesicle tubular clusters (VTCs) by direct interaction with the small GTPase RAB2, where this kinase phosphorylates glyceraldehydes-3-phosphate dehydrogenase (GAPD/GAPDH) and plays a role in microtubule dynamics in the early secretory pathway. This kinase is found to be necessary for BCL-ABL-mediated resistance to drug-induced apoptosis and therefore protects leukemia cells against drug-induced apoptosis.

Molecular Weight: 68262

Gene ID: 5584

NCBI Accession: NP_002731

UniProt: P41743

Pathways: Neurotrophin Signaling Pathway, Cell-Cell Junction Organization, Tube Formation