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CD14 antibody (N-Term)

Name: CD14 antibody (N-Term)
SKU: ABIN390260
Price: 429 USD
Availability: InStock

CD14 Reactivity: Human FACS, WB, IF, IHC (p) Host: Rabbit Polyclonal RB14105 unconjugated

Overlay histogram showing Jurkat cells stained with A(green line). (CD14 antibody (N-Term))

FACS

Immunofluorescent analysis of 4 % paraformaldehyde-fixed, 0. (CD14 antibody (N-Term))

All lanes : Anti-CD14 Antibody (N-term) at 1:2000 dilution Lane 1: Human lung tissue lysate Lane 2: Human liver tissue lysate Lane 3: A549 whole cell lysate Lysates/proteins at 20 μg per lane. (CD14 antibody (N-Term))

All lanes : Anti-CD14 Antibody (N-term) at 1:1000 dilution Lane 1: Human lung tissue lysate Lane 2: Human liver tissue lysate Lysates/proteins at 20 μg per lane. (CD14 antibody (N-Term))

All lanes : Anti-CD14 Antibody (N-term) at 1:1000 dilution Lane 1: THP-1 whole cell lysate Lane 2: Human lung tissue lysate Lane 3: Human liver tissue lysate Lane 4: A549 whole cell lysate Lysates/proteins at 20 μg per lane. (CD14 antibody (N-Term))

All lanes : Anti-CD14 Antibody (N-term) at 1:2000 dilution Lane 1: Human liver lysate Lane 2: Human lung lysate Lysates/proteins at 20 μg per lane. (CD14 antibody (N-Term))

Immunohistochemical analysis of paraffin-embedded human liver tissue using A performed on the Leica® BOND RXm. (CD14 antibody (N-Term))

IHC (p)

7 images

(1 reference)

Catalog No. ABIN390260

Datasheet as PDF

Target See all CD14 Antibodies

CD14
Binding Specificity
9

6

5

4

3

3

3

3

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 54-83, N-Term
Reactivity
221

38

30

25

22

18

16

15

14

13

13

10

9

9

8

8

8

5

2

2

2

1

1

1
Human
Host
192

51

16

3

1
Rabbit
Compatible Secondaries
Clonality
210

53
Polyclonal
Conjugate
107

30

22

17

13

5

5

4

4

4

4

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This CD14 antibody is un-conjugated
Application
196

78

56

50

45

43

20

13

12

11

7

4

4

3

3

3

2

2

2

1

1

1

1

1

1

1
Flow Cytometry (FACS), Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purification

This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen

This CD14 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 54-83 amino acids from the N-terminal region of human CD14.

Clone

RB14105

Isotype

IgG
anti-CD14 (CD14) (AA 20-214) antibody
CD14 Reactivity: Human, Mouse FACS, WB, IHC, ELISA Host: Mouse Monoclonal 4B4F12 unconjugated

anti-CD14 (CD14) (AA 20-214) antibody
CD14 Reactivity: Human WB, IHC, ELISA, ICC Host: Mouse Monoclonal 1H5D8 unconjugated

anti-CD14 (CD14) antibody
CD14 Reactivity: Human IHC, ELISA Host: Mouse Monoclonal 1A3B6 unconjugated

anti-CD14 (CD14) (AA 20-345) antibody
CD14 Reactivity: Human FACS, WB, IHC, ELISA, IF Host: Mouse Monoclonal 14G1F3 unconjugated

anti-CD14 (CD14) (AA 230-345) antibody
CD14 Reactivity: Human FACS, WB, IHC, ELISA Host: Mouse Monoclonal 1D1F8 unconjugated

anti-CD14 (CD14) (AA 25-148) antibody
CD14 Reactivity: Human FACS, WB, IHC, ISt, StM Host: Mouse Monoclonal LPSR-2385 unconjugated

anti-CD14 (CD14) antibody
CD14 Reactivity: Human, Non-Human Primate FACS, WB, ELISA, IP Host: Mouse Monoclonal MEM-18 unconjugated

anti-CD14 (CD14) antibody
CD14 Reactivity: Human, Non-Human Primate FACS, WB, ELISA, IP, Func Host: Mouse Monoclonal MEM-18 unconjugated

anti-CD14 (CD14) (AA 201-300) antibody
CD14 Reactivity: Human, Mouse, Rat, Dog, Sheep, Rabbit WB, ELISA, IHC (p), ICC, IHC (fro), IF (cc), IF (p) Host: Rabbit Polyclonal unconjugated

anti-CD14 (CD14) antibody (Biotin)
CD14 Reactivity: Human, Non-Human Primate FACS, WB, ELISA, IP Host: Mouse Monoclonal MEM-18 Biotin

Application Notes

IF: 1:25. WB: 1:2000. WB: 1:1000. WB: 1:1000. WB: 1:2000. IHC-P-Leica: 1:1000. FC: 1:25

Restrictions

For Research Use only
Format

Liquid

Buffer

Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

4 °C,-20 °C

Storage Comment

Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date

6 months
Ishida, Akita, Mori, Tanida, Toda, Inoue, Nakada: "Negative regulation of Toll-like receptor-4 signaling through the binding of glycosylphosphatidylinositol-anchored glycoprotein, CD14, with the sialic acid-binding lectin, CD33." in: The Journal of biological chemistry, Vol. 289, Issue 36, pp. 25341-50, (2014) (PubMed).
Target

CD14

Alternative Name

CD14 (CD14 Products)

Synonyms

CD14 molecule antibody, CD14 antigen antibody, CD14 antibody, Cd14 antibody

Background

CD14 is a surface protein preferentially expressed on monocytes/macrophages. It binds lipopolysaccharide binding protein and recently has been shown to bind apoptotic cells.

Molecular Weight

40076

Gene ID

929

NCBI Accession

NP_000582, NP_001035110, NP_001167575, NP_001167576

UniProt

P08571

Pathways

TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades