MAGEA3 antibody (C-Term)

Catalog No. ABIN390107

Product Details anti-MAGEA3 Antibody

Target: MAGEA3 (Melanoma Antigen Family A, 3 (MAGEA3))

Binding Specificity: AA 283-314, C-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MAGEA3 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogen: This MAGEA3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 283-314 amino acids from the C-terminal region of human MAGEA3.

Clone: RB2084

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:1000. IHC-P: 1:10~50. FC: 1:10~50

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

References for anti-MAGEA3 antibody (ABIN390107)

Curioni-Fontecedro, Knights, Tinguely, Nuber, Schneider, Thomson, von Boehmer, Bossart, Pahlich, Gehring, Moch, Renner, Knuth, Zippelius: "MAGE-C1/CT7 is the dominant cancer-testis antigen targeted by humoral immune responses in patients with multiple myeloma." in: Leukemia, Vol. 22, Issue 8, pp. 1646-8, (2008) (PubMed).

Dubovsky, Albertini, McNeel: "MAD-CT-2 identified as a novel melanoma cancer-testis antigen using phage immunoblot analysis." in: Journal of immunotherapy (Hagerstown, Md. : 1997), Vol. 30, Issue 7, pp. 675-83, (2007) (PubMed).

Kondo, Zhu, Asa, Ezzat: "The cancer/testis antigen melanoma-associated antigen-A3/A6 is a novel target of fibroblast growth factor receptor 2-IIIb through histone H3 modifications in thyroid cancer." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 13, Issue 16, pp. 4713-20, (2007) (PubMed).

Target Details for MAGEA3

Target: MAGEA3 (Melanoma Antigen Family A, 3 (MAGEA3))

Alternative Name: MAGEA3 (MAGEA3 Products)

Synonyms: CT1.3 antibody, HIP8 antibody, HYPD antibody, MAGE3 antibody, MAGEA6 antibody, Mage-a3 antibody, MAGEA3 antibody, MAGE family member A3 antibody, melanoma antigen, family A, 3 antibody, melanoma antigen family A, 3 antibody, MAGEA3 antibody, Magea3 antibody

Background: MAGEA3 is a member of the MAGEA gene family. The members of this family have their entire coding sequences located in the last exon, and the encoded proteins show 50 to 80 % sequence identity between each other. The promoters and first exons of the MAGEA genes show considerable variability, suggesting that the existence of this gene family enables the same function to be expressed under different transcriptional controls. The MAGEA genes are expressed at a high level in a number of tumors of various histologic types, and are silent in normal tissues with the exception of testis and placenta. The MAGEA genes are clustered on chromosome Xq28. They may be implicated in some hereditary disorders, such as dyskeratosis congenita.

Molecular Weight: 34747

Gene ID: 4102

NCBI Accession: NP_005353

UniProt: P43357