ACE2 antibody (C-Term)

Catalog No. ABIN390049

Product Details anti-ACE2 Antibody

Target: ACE2 (Angiotensin I Converting Enzyme 2 (ACE2))

Binding Specificity: AA 773-805, C-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ACE2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogen: This ACE2 (SARS Receptor) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 773-805 amino acids from the C-terminal region of human ACE2 (SARS Receptor).

Clone: RB4819

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:1000. WB: 1:1000. IHC-P: 1:50~100. IHC-P: 1:50~100

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

References for anti-ACE2 antibody (ABIN390049)

Heurich, Hofmann-Winkler, Gierer, Liepold, Jahn, Pöhlmann: "TMPRSS2 and ADAM17 cleave ACE2 differentially and only proteolysis by TMPRSS2 augments entry driven by the severe acute respiratory syndrome coronavirus spike protein." in: Journal of virology, Vol. 88, Issue 2, pp. 1293-307, (2013) (PubMed).

Target Details for ACE2

Target: ACE2 (Angiotensin I Converting Enzyme 2 (ACE2))

Alternative Name: ACE2 (SARS Receptor) (ACE2 Products)

Synonyms: ACE1 antibody, CD143 antibody, DCP antibody, DCP1 antibody, ICH antibody, MVCD3 antibody, ACEH antibody, 2010305L05Rik antibody, zgc:92514 antibody, angiotensin I converting enzyme antibody, angiotensin I converting enzyme 2 antibody, angiotensin I converting enzyme (peptidyl-dipeptidase A) 2 antibody, ACE antibody, ACE2 antibody, Ace2 antibody, ace2 antibody

Background: ACE2 cDNA encodes a deduced 805-amino acid protein containing a potential 17-amino acid N-terminal signal peptide and a putative 22-amino acid C-terminal membrane anchor. It also possesses a zinc metalloprotease consensus sequence and a conserved glutamine residue that may function as a third zinc ligand. ACE2 is expressed predominantly in vascular endothelial cells of the heart and kidney. ACE converts angiotensin I to angiotensin II, ACE2 converts angiotensin I to angiotensin 1-9, which has 9 amino acids. Angiotensin II is a potent blood vessel constrictor, while angiotensin 1-9 does not impact blood vessels but is cleaved by ACE to a shorter peptide, angiotensin 1-7, which is a blood vessel dilator. Spike (S) proteins of coronaviruses, including the SARS coronavirus, bind with cellular receptors to mediate infection of target cells. ACE2 binds the S1 domain of the SARS coronavirus S protein. SARS coronavirus replicates efficiently on ACE2-transfected but not mock-transfected 293T cells. Anti-ACE2 but not anti-ACE1 antibody blocks viral replication on Vero E6 cells. It has been proposed that ACE2 is a functional receptor for SARS coronavirus.

Molecular Weight: 92463

Gene ID: 59272

NCBI Accession: NP_068576

UniProt: Q9BYF1

Pathways: ACE Inhibitor Pathway, Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones, Feeding Behaviour