This antibody is purified through a protein A column, followed by peptide affinity purification.
Immunogen
This Phospho-RAF1-S259 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S259 of human RAF1.
RAF1
Reactivity: Human, Mouse, Rat
WB, IHC, IF
Host: Rabbit
Polyclonal
unconjugated
Application Notes
DB: 1:500
Restrictions
For Research Use only
Format
Liquid
Buffer
Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C,-20 °C
Storage Comment
Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date
6 months
Duan, Cobb: "Calcineurin increases glucose activation of ERK1/2 by reversing negative feedback." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 107, Issue 51, pp. 22314-9, (2010) (PubMed).
Raf-1 is a MAP kinase kinase kinase (MAP3K) which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated Raf-1 can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2 which in turn phosphorylate to activate the serine/threonine specific protein kinases ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration.