LC3C antibody (pSer12)

Catalog No. ABIN389699

Product Details anti-LC3C Antibody

Target: LC3C (MAP1LC3C) (Microtubule-Associated Protein 1 Light Chain 3 gamma (MAP1LC3C))

Binding Specificity: pSer12

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LC3C antibody is un-conjugated

Application: Western Blotting (WB), Dot Blot (DB)

Predicted Reactivity: Zf, B, M, Rat

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This LC3C Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S12 of human LC3C.

Clone: RB10838-RB16769

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:1000. WB: 1:1000. WB: 1:1000. WB: 1:1000. DB: 1:500

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

References for anti-LC3C antibody (ABIN389699)

Tanaka, Koyama, Sakurai, Kamiyoshi, Ichikawa-Shindo, Kawate, Liu, Xian, Imai, Zhai, Hirabayashi, Owa, Yamauchi, Igarashi, Taniguchi, Shindo: "The endothelial adrenomedullin-RAMP2 system regulates vascular integrity and suppresses tumour metastasis." in: Cardiovascular research, Vol. 111, Issue 4, pp. 398-409, (2017) (PubMed).

Xie, Kang, Tang: "Assessment of Posttranslational Modifications of ATG proteins." in: Methods in enzymology, Vol. 587, pp. 171-188, (2017) (PubMed).

Li, Liu, Wang, Wang, Zhao, Su, Zhang, Zhang, Xu, Zhao, Miao: "Identification of a small molecule targeting annexin A7." in: Biochimica et biophysica acta, Vol. 1833, Issue 9, pp. 2092-9, (2013) (PubMed).

Colecchia, Strambi, Sanzone, Iavarone, Rossi, DallArmi, Piccioni, Verrotti di Pianella, Chiariello: "MAPK15/ERK8 stimulates autophagy by interacting with LC3 and GABARAP proteins." in: Autophagy, Vol. 8, Issue 12, pp. 1724-40, (2012) (PubMed).

Su, Chao, Huang, Weng, Jeng, Lai: "Rab5 and class III phosphoinositide 3-kinase Vps34 are involved in hepatitis C virus NS4B-induced autophagy." in: Journal of virology, Vol. 85, Issue 20, pp. 10561-71, (2011) (PubMed).

Cherra, Kulich, Uechi, Balasubramani, Mountzouris, Day, Chu: "Regulation of the autophagy protein LC3 by phosphorylation." in: The Journal of cell biology, Vol. 190, Issue 4, pp. 533-9, (2010) (PubMed).

Target Details for LC3C

Target: LC3C (MAP1LC3C) (Microtubule-Associated Protein 1 Light Chain 3 gamma (MAP1LC3C))

Alternative Name: LC3C (MAP1LC3C Products)

Synonyms: MAP1LC3C antibody, MGC154878 antibody, ATG8J antibody, LC3C antibody, microtubule associated protein 1 light chain 3 gamma antibody, microtubule associated protein 1 light chain 3 gamma L homeolog antibody, MAP1LC3C antibody, map1lc3c antibody, map1lc3c.L antibody

Background: MAP1A and MAP1B are microtubule-associated proteins which mediate the physical interactions between microtubules and components of the cytoskeleton. These proteins are involved in formation of autophagosomal vacuoles (autophagosomes). MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. MAP1LC3a is one of the light chain subunits and can associate with either MAP1A or MAP1B. The precursor molecule is cleaved by APG4B/ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II. Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole).

Molecular Weight: 14272

Gene ID: 84557

NCBI Accession: NP_115903, NP_852610

UniProt: Q9H492

Pathways: Autophagy