Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Predicted Reactivity
M, Pig, Rat
Purification
This antibody is purified through a protein A column, followed by peptide affinity purification.
Immunogen
This Syk Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding Y525/526 of human Syk.
Köhler, Fuhr, Dinekov: "Distribution of spleen tyrosine kinase and tau phosphorylated at tyrosine 18 in a mouse model of tauopathy and in the human hippocampus." in: Brain research, Vol. 1677, pp. 1-13, (2018) (PubMed).
Greenblatt, Park, Oh, Kim, Shin, Lee, Lee, Singh, Lee, Hu, Xiao, Charles, Penninger, Lotinun, Baron, Ghosh, Shim: "CHMP5 controls bone turnover rates by dampening NF-?B activity in osteoclasts." in: The Journal of experimental medicine, Vol. 212, Issue 8, pp. 1283-301, (2015) (PubMed).
Nishikawa, Takahashi, Nakamori, Hosomi, Maruyama, Miyazaki, Izumi, Matsumoto: "The identification of raft-derived tau-associated vesicles that are incorporated into immature tangles and paired helical filaments." in: Neuropathology and applied neurobiology, (2015) (PubMed).
Satoh, Tabunoki, Ishida, Yagishita, Jinnai, Futamura, Kobayashi, Toyoshima, Yoshioka, Enomoto, Arai, Saito, Arima: "Phosphorylated Syk expression is enhanced in Nasu-Hakola disease brains." in: Neuropathology : official journal of the Japanese Society of Neuropathology, Vol. 32, Issue 2, pp. 149-57, (2012) (PubMed).
Syk is a positive effector of BCR-stimulated responses. This protein couples the B-cell antigen receptor (BCR) to the mobilization of calcium ion either through a phosphoinositide 3-kinase-dependent pathway, when not phosphorylated on tyrosines of the linker region, or through a phospholipase C-gamma-dependent pathway, when phosphorylated on Tyr-342 and Tyr-346. Thus the differential phosphorylation of Syk can determine the pathway by which BCR is coupled to the regulation of intracellular calcium ion.