Separase antibody (pSer1126)

Catalog No. ABIN389654

The Rabbit Polyclonal anti-Separase antibody has been validated for IHC (p) and DB. It is suitable to detect Separase in samples from Human. There is 1 publication available.

Quick Overview for Separase antibody (pSer1126) (ABIN389654)

Target: Separase (ESPL1) (Extra Spindle Poles Like 1 (ESPL1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Separase antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Dot Blot (DB)

Clone: RB7943

Product Details anti-Separase Antibody

Binding Specificity: pSer1126

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This SEPARIN Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S1126 of human SEPARIN.

Isotype: Ig Fraction

Application Details

Application Notes: IHC-P: 1:50~100. DB: 1:500

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

References for anti-Separase antibody (ABIN389654)

Haaß, Stehle, Nittka, Giehl, Schrotz-King, Fabarius, Hofmann, Seifarth: "The proteolytic activity of separase in BCR-ABL-positive cells is increased by imatinib." in: PLoS ONE, Vol. 7, Issue 8, pp. e42863, (2012) (PubMed).

Target Details for Separase

Target: Separase (ESPL1) (Extra Spindle Poles Like 1 (ESPL1))

Alternative Name: SEPARIN

Background: The metaphase-to-anaphase transition is the final discrete event in duplication and separation of the genetic material of the cell. Its timing is regulated by the activation of the anaphase-promoting complex (APC). In both budding and fission yeast, the degradation of the Pds1 or Cut2 protein, respectively, is required for the onset of sister chromatid separation. Both proteins are APC substrates. Pds1 and Cut2 proteins associate with the yeast separin proteins Esp1 and Cut1, respectively, and prevent the separins from promoting chromatid separation. Pds1 and Cut2 are also called anaphase inhibitors or securins

Molecular Weight: 233175

Gene ID: 9700

NCBI Accession: NP_036423

UniProt: Q14674