Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Specificity
This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S612 of human RB
Purification
This antibody is purified through a protein G column, followed by dialysis against PBS.
Immunogen
This RB Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S612 of human RB.
RB1
Reactivity: Human
WB, IHC
Host: Rabbit
Monoclonal
unconjugated
Application Notes
WB = 1:1000, IHC (p) = 1:50-100
Restrictions
For Research Use only
Format
Liquid
Concentration
0.38 mg/mL
Buffer
PBS with 0.09 % (W/V) sodium azide
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C/-20 °C
Storage Comment
Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date
6 months
Rizzolio, Lucchetti, Caligiuri, Marchesi, Caputo, Klein-Szanto, Bagella, Castronovo, Giordano: "Retinoblastoma tumor-suppressor protein phosphorylation and inactivation depend on direct interaction with Pin1." in: Cell death and differentiation, Vol. 19, Issue 7, pp. 1152-61, (2012) (PubMed).