RAD9A antibody (pSer328)

Catalog No. ABIN389633

Product Details anti-RAD9A Antibody

Target: RAD9A (RAD9 Homolog A (S. Pombe) (RAD9A))

Binding Specificity: pSer328

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RAD9A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Dot Blot (DB)

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This RAD9 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S328 of human RAD9.

Clone: RB7115

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:1000. IHC-P: 1:50~100. DB: 1:500

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

References for anti-RAD9A antibody (ABIN389633)

Awate, De Benedetti: "TLK1B mediated phosphorylation of Rad9 regulates its nuclear/cytoplasmic localization and cell cycle checkpoint." in: BMC molecular biology, Vol. 17, pp. 3, (2016) (PubMed).

Ronald, Awate, Rath, Carroll, Galiano, Dwyer, Kleiner-Hancock, Mathis, Vigod, De Benedetti: "Phenothiazine Inhibitors of TLKs Affect Double-Strand Break Repair and DNA Damage Response Recovery and Potentiate Tumor Killing with Radiomimetic Therapy." in: Genes & cancer, Vol. 4, Issue 1-2, pp. 39-53, (2013) (PubMed).

Target Details for RAD9A

Target: RAD9A (RAD9 Homolog A (S. Pombe) (RAD9A))

Alternative Name: RAD9 (RAD9A Products)

Synonyms: RAD9 antibody, Rad9 antibody, rad9a antibody, MGC89062 antibody, XRad9 antibody, rad9 antibody, RAD9 checkpoint clamp component A antibody, RAD9 checkpoint clamp component B antibody, RAD9 checkpoint clamp component A L homeolog antibody, RAD9A antibody, Rad9a antibody, rad9a antibody, rad9b antibody, rad9a.L antibody

Background: Rad9 is highly similar to Schizosaccharomyces pombe rad9, a cell cycle checkpoint protein required for cell cycle arrest and DNA damage repair in response to DNA damage. This protein is found to possess 3' to 5' exonuclease activity, which may contribute to its role in sensing and repairing DNA damage. It forms a checkpoint protein complex with RAD1 and HUS1. This complex is recruited by checkpoint protein RAD17 to the sites of DNA damage, which is thought to be important for triggering the checkpoint-signaling cascade.

Molecular Weight: 42547

Gene ID: 5883

NCBI Accession: NP_001230153, NP_004575

UniProt: Q99638

Pathways: Positive Regulation of Response to DNA Damage Stimulus