CDKN1B antibody (pThr187)

Catalog No. ABIN389617

Product Details anti-CDKN1B Antibody

Target: CDKN1B (Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B))

Binding Specificity: pThr187

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CDKN1B antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Predicted Reactivity: Ha

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This p27Kip1 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding T187 of human p27Kip1.

Clone: RB07331

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:1000. IHC-P: 1:50~100

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

References for anti-CDKN1B antibody (ABIN389617)

van der Sligte, Scherpen, Ter Elst, Guryev, van Leeuwen, de Bont: "Effect of IKZF1 deletions on signal transduction pathways in Philadelphia chromosome negative pediatric B-cell precursor acute lymphoblastic leukemia (BCP-ALL)." in: Experimental hematology & oncology, Vol. 4, pp. 23, (2015) (PubMed).

Fang, Zhang, Dizeyi, Chen, Wang, Swanson, Cai, Balk, Yuan: "Androgen Receptor Enhances p27 Degradation in Prostate Cancer Cells through Rapid and Selective TORC2 Activation." in: The Journal of biological chemistry, Vol. 287, Issue 3, pp. 2090-8, (2012) (PubMed).

Le, Mao, He, Claret, Xia, Ahmed, Hung, Siddik, Bast: "The role of p27(Kip1) in dasatinib-enhanced paclitaxel cytotoxicity in human ovarian cancer cells." in: Journal of the National Cancer Institute, Vol. 103, Issue 18, pp. 1403-22, (2011) (PubMed).

Target Details for CDKN1B

Target: CDKN1B (Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B))

Alternative Name: p27Kip1 (CDKN1B Products)

Background: P27Kip1 is a cyclin-dependent kinase inhibitor, which shares a limited similarity with CDK inhibitor CDKN1A/p21. The encoded protein binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controls the cell cycle progression at G1. The degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state.

Molecular Weight: 22073

Gene ID: 1027

NCBI Accession: NP_004055

UniProt: P46527

Pathways: Cell Division Cycle, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Negative Regulation of Hormone Secretion, Sensory Perception of Sound, Mitotic G1-G1/S Phases, DNA Replication, Positive Regulation of Endopeptidase Activity, Synthesis of DNA, Autophagy