PCNA antibody (C-Term)

Catalog No. ABIN389344

Product Details anti-PCNA Antibody

Target: PCNA (Proliferating Cell Nuclear Antigen (PCNA))

Binding Specificity: AA 231-261, C-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PCNA antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Predicted Reactivity: B, Ha, Pr, Rat

Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogen: This PCNA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 231-261 amino acids from the C-terminal region of human PCNA.

Clone: RB17486

Isotype: Ig Fraction

Application Details

Application Notes: IF: 1:10~50. IF: 1:10~50. WB: 1:1000. WB: 1:1000. IHC-P: 1:10~50. IHC-P: 1:10~50

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

References for anti-PCNA antibody (ABIN389344)

Deng, Xu, Zou, Shen, Peng, Liu, Engelhardt, Yan, Qiu: "DNA Damage Signaling Is Required for Replication of Human Bocavirus 1 DNA in Dividing HEK293 Cells." in: Journal of virology, Vol. 91, Issue 1, (2017) (PubMed).

Lei, Li, Luo: "Liuweiwuling tablets attenuate acetaminophen-induced acute liver injury and promote liver regeneration in mice." in: World journal of gastroenterology : WJG, Vol. 21, Issue 26, pp. 8089-95, (2015) (PubMed).

Ding, Ko, Pehar, Kotch, Peters, Luo, Salamat, Puglielli: "Biochemical inhibition of the acetyltransferases ATase1 and ATase2 reduces ?-secretase (BACE1) levels and A? generation." in: The Journal of biological chemistry, Vol. 287, Issue 11, pp. 8424-33, (2012) (PubMed).

Ma, He, Zhang, Fei, Niu, Wang, Ding, Xu, Chen, Zhu: "DNA methylation-regulated miR-193a-3p dictates resistance of hepatocellular carcinoma to 5-fluorouracil via repression of SRSF2 expression." in: The Journal of biological chemistry, Vol. 287, Issue 8, pp. 5639-49, (2012) (PubMed).

Li, Li, Zhang, Bai, Wang, Zhao, Jonas: "Lentiviral vector-mediated PAX6 overexpression promotes growth and inhibits apoptosis of human retinoblastoma cells." in: Investigative ophthalmology & visual science, Vol. 52, Issue 11, pp. 8393-400, (2011) (PubMed).

Target Details for PCNA

Target: PCNA (Proliferating Cell Nuclear Antigen (PCNA))

Alternative Name: PCNA (PCNA Products)

Background: PCNA is found in the nucleus and is a cofactor of DNA polymerase delta. This protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway.

Molecular Weight: 28769

Gene ID: 5111

NCBI Accession: NP_002583, NP_872590

UniProt: P12004

Pathways: Telomere Maintenance, DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA, Autophagy