LGR5 antibody (AA 689-719)

Catalog No. ABIN389218

Product Details anti-LGR5 Antibody

Target: LGR5 (Leucine-Rich Repeat Containing G Protein-Coupled Receptor 5 (LGR5))

Binding Specificity: AA 689-719

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LGR5 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS)

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This LGR5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 689-719 amino acids from human LGR5.

Clone: RB14211

Isotype: IgG

Application Details

Application Notes: WB: 1:1000. IF: 1:100. FC: 1:25

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots.

Expiry Date: 6 months

References for anti-LGR5 antibody (ABIN389218)

Huch, Gehart, van Boxtel, Hamer, Blokzijl, Verstegen, Ellis, van Wenum, Fuchs, de Ligt, van de Wetering, Sasaki, Boers, Kemperman, de Jonge, Ijzermans, Nieuwenhuis, Hoekstra, Strom, Vries et al.: "Long-term culture of genome-stable bipotent stem cells from adult human liver. ..." in: Cell, Vol. 160, Issue 1-2, pp. 299-312, (2015) (PubMed).

Ying, Tsujii, Kondo, Hayashi, Kato, Akasaka, Inoue, Shiraishi, Inoue, Hiyama, Tsujii, Maekawa, Kawai, Fujinaga, Araki, Shinzaki, Watabe, Nishida, Iijima, Takehara: "The effectiveness of an anti-human IL-6 receptor monoclonal antibody combined with chemotherapy to target colon cancer stem-like cells." in: International journal of oncology, Vol. 46, Issue 4, pp. 1551-9, (2015) (PubMed).

Singh, Mallela, Cornuet, Reifler, Chervenak, West, Wong, Nasonkin: "Characterization of Three-Dimensional Retinal Tissue Derived from Human Embryonic Stem Cells in Adherent Monolayer Cultures." in: Stem cells and development, Vol. 24, Issue 23, pp. 2778-95, (2015) (PubMed).

Jardé, Kass, Staples, Lescesen, Carne, Oliva, McMurrick, Abud: "ERBB3 Positively Correlates with Intestinal Stem Cell Markers but Marks a Distinct Non Proliferative Cell Population in Colorectal Cancer." in: PLoS ONE, Vol. 10, Issue 9, pp. e0138336, (2015) (PubMed).

Bu, Zheng, Zhang, Li, Sun, Dong, Wu, Wu, Wang, Cheng, Xing, Li, Du, Ji: "LGR5 is a promising biomarker for patients with stage I and II gastric cancer." in: Chinese journal of cancer research = Chung-kuo yen cheng yen chiu, Vol. 25, Issue 1, pp. 79-89, (2013) (PubMed).

Zheng, Sun, Bu, Zhang, Li, Wu, Wu, Wang, Cheng, Xing, Du, Ji: "Intestinal stem cell marker LGR5 expression during gastric carcinogenesis." in: World journal of gastroenterology : WJG, Vol. 19, Issue 46, pp. 8714-21, (2013) (PubMed).

Maddox, Shakya, South, Shelton, Andersen, Chidester, Kang, Gligorich, Jones, Spangrude, Welm, Tantin: "Transcription factor Oct1 is a somatic and cancer stem cell determinant." in: PLoS genetics, Vol. 8, Issue 11, pp. e1003048, (2012) (PubMed).

Judah, Rudkouskaya, Wilson, Carter, Dagnino: "Multiple roles of integrin-linked kinase in epidermal development, maturation and pigmentation revealed by molecular profiling." in: PLoS ONE, Vol. 7, Issue 5, pp. e36704, (2012) (PubMed).

Takahashi, Ishii, Nishida, Takemasa, Mizushima, Ikeda, Yokobori, Mimori, Yamamoto, Sekimoto, Doki, Mori: "Significance of Lgr5(+ve) cancer stem cells in the colon and rectum." in: Annals of surgical oncology, Vol. 18, Issue 4, pp. 1166-74, (2011) (PubMed).

Satoh, Obayashi, Tabunoki, Wakana, Kim: "Stable expression of neurogenin 1 induces LGR5, a novel stem cell marker, in an immortalized human neural stem cell line HB1.F3." in: Cellular and molecular neurobiology, Vol. 30, Issue 3, pp. 415-26, (2010) (PubMed).

Target Details for LGR5

Target: LGR5 (Leucine-Rich Repeat Containing G Protein-Coupled Receptor 5 (LGR5))

Alternative Name: LGR5/GPR49 (LGR5 Products)

Synonyms: lgr5-a antibody, lgr5a antibody, FEX antibody, Gpr49 antibody, GPR49 antibody, GPR67 antibody, GRP49 antibody, HG38 antibody, leucine-rich repeat containing G protein-coupled receptor 5 L homeolog antibody, leucine rich repeat containing G protein coupled receptor 5 antibody, leucine rich repeat containing G protein-coupled receptor 5 antibody, lgr5.L antibody, Lgr5 antibody, LGR5 antibody

Background: LGR5/GPR49 is an orphan receptor. It may be an important receptor for signals controlling growth and differentiation of specific embryonic tissues. Stem cell marker of the intestinal epithelium and the hair follicle. Target gene of Wnt signaling. Expressed in skeletal muscle, placenta, spinal cord, and various region of brain. Expressed at the base of crypts in colonic and small mucosa stem cells. In premalignant cancer expression is not restricted to the cript base. Overexpressed in cancers of the ovary, colon and liver.

Molecular Weight: 99998

Gene ID: 8549

NCBI Accession: NP_001264155, NP_003658

UniProt: O75473

Pathways: WNT Signaling