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Vimentin antibody (C-Term)

Name: Vimentin antibody (C-Term)
SKU: ABIN389212
Price: 429 USD
Availability: InStock

VIM Reactivity: Human WB, IF, FACS, IHC (p) Host: Rabbit Polyclonal RB16691 unconjugated

Anti-Vimentin Antibody (C-term) at 1:1000 dilution + U-2OS whole cell lysate Lysates/proteins at 20 μg per lane. (Vimentin antibody (C-Term))

Vimentin Antibody (C-term) (ABIN389212 and ABIN2839367) immunohistochemistry analysis in formalin fixed and paraffin embedded human colon tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. (Vimentin antibody (C-Term))

IHC (p)

Fluorescent confocal image of SY5Y cells stained with Vimentin (C-term) antibody. (Vimentin antibody (C-Term))

Western blot analysis of Vimentin antibody (C-term) (ABIN389212 and ABIN2839367) in NCI- cell line lysates (35 μg/lane). (Vimentin antibody (C-Term))

Vimentin Antibody (C-term) (ABIN389212 and ABIN2839367) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram). (Vimentin antibody (C-Term))

FACS

5 images

(2 references)

Catalog No. ABIN389212

Datasheet as PDF

Target See all Vimentin (VIM) Antibodies

Vimentin (VIM)
Binding Specificity
32

18

16

16

15

14

11

9

7

7

4

3

3

3

3

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 430-457, C-Term
Reactivity
341

134

131

60

60

49

42

37

29

21

20

13

13

8

8

6

6

3

3

2

1

1

1

1

1

1

1

1

1
Human
Host
194

130

11

6

6

5

2
Rabbit
Compatible Secondaries
Clonality
210

143
Polyclonal
Conjugate
203

19

14

14

10

7

4

4

4

4

4

4

4

4

4

4

4

4

4

4

4

4

4

3

2

2

2

2

2

2

2

1

1
This Vimentin antibody is un-conjugated
Application
285

129

106

100

95

63

46

40

39

33

23

19

8

5

4

4

3

2

2

2

1

1

1

1

1

1

1

1
Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Predicted Reactivity

X, B, C, Ha, Pr, M, Pig, Rat

Purification

This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogen

This Vimentin antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 430-457 amino acids from the C-terminal region of human Vimentin.

Clone

RB16691

Isotype

Ig Fraction

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KO Validated VIM Reactivity: Human WB, IHC, ELISA, IF, IHC (p), ICC, IP, IHC (fro) Host: Rabbit Polyclonal unconjugated

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VIM Reactivity: Mammalian WB, FACS, IHC (p), ICC Host: Mouse Monoclonal VI-01 unconjugated

anti-Vimentin (VIM) antibody
VIM Reactivity: Human, Mouse, Rat, Pig, Chicken WB, IHC (p), ICC, IP Host: Mouse Monoclonal VI-10 unconjugated

anti-Vimentin (VIM) antibody
VIM Reactivity: Human, Mouse WB, IF, FACS, IHC (p) Host: Rabbit Polyclonal RB41034 unconjugated

anti-Vimentin (VIM) (AA 1-200) antibody
VIM Reactivity: Human WB, IHC, IF Host: Rabbit Polyclonal unconjugated

anti-Vimentin (VIM) antibody
VIM Reactivity: Human WB, IHC, ICC, IP Host: Mouse Monoclonal C8 unconjugated

anti-Vimentin (VIM) (AA 2-466) antibody
VIM Reactivity: Human, Mouse, Rat WB, IHC, ELISA, FACS Host: Mouse Monoclonal 3A1F2 unconjugated

anti-Vimentin (VIM) antibody
VIM Reactivity: Human WB, IHC, StM Host: Rabbit Monoclonal VIM-1937R unconjugated Recombinant Antibody

anti-Vimentin (VIM) antibody
VIM Reactivity: Human, Pig, Cow, Chicken, Dog, Cat, Goat WB, IHC, FACS, StM Host: Mouse Monoclonal VM452 unconjugated

Application Notes

IF: 1:100. WB: 1:1000. WB: 1:1000. IHC-P: 1:10~50. FC: 1:10~50

Restrictions

For Research Use only
Format

Liquid

Buffer

Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

4 °C,-20 °C

Storage Comment

Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date

6 months
Liu, Li, Li, Yang: "Pituitary tumor transforming gene PTTG2 induces psoriasis by regulating vimentin and E-cadherin expression." in: International journal of clinical and experimental pathology, Vol. 8, Issue 9, pp. 10887-93, (2016) (PubMed).

Dai, Duan, Duan, Zhou, He, Tu, Shen: "Down-regulation of TCF21 by hypermethylation induces cell proliferation, migration and invasion in colorectal cancer." in: Biochemical and biophysical research communications, (2015) (PubMed).
Target

Vimentin (VIM)

Alternative Name

Vimentin (VIM Products)

Synonyms

CTRCT30 antibody, cb28 antibody, vime antibody, vim antibody, vim1 antibody, vim2 antibody, VIM antibody, Vimentin antibody, vim4 antibody, vimentin antibody, vimentin L homeolog antibody, vimentin S homeolog antibody, VIM antibody, Vim antibody, vim antibody, vim.L antibody, vim.S antibody

Background

Along with the microfilaments (actins) and microtubules (tubulins), the intermediate filaments represent a third class of well-characterized cytoskeletal elements. The subunits display a tissue-specific pattern of expression. Desmin is the subunit specific for muscle and vimentin the subunit specific for mesenchymal tissue.

Molecular Weight

53652

Gene ID

7431

NCBI Accession

NP_003371

UniProt

P08670

Pathways

Caspase Cascade in Apoptosis