CBX4 antibody (N-Term)

Catalog No. ABIN389086

Product Details anti-CBX4 Antibody

Target: CBX4 (Chromobox Homolog 4 (CBX4))

Binding Specificity: AA 80-110, N-Term

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CBX4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This PC2 (CBX4) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 80-110 amino acids from the N-terminal region of human PC2 (CBX4).

Clone: RB2931

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:1000. IHC-P: 1:50~100

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

References for anti-CBX4 antibody (ABIN389086)

Oh, Kim, Mouradian, Chung: "Human Polycomb protein 2 promotes ?-synuclein aggregate formation through covalent SUMOylation." in: Brain research, Vol. 1381, pp. 78-89, (2011) (PubMed).

Yang, Sharrocks: "The SUMO E3 ligase activity of Pc2 is coordinated through a SUMO interaction motif." in: Molecular and cellular biology, Vol. 30, Issue 9, pp. 2193-205, (2010) (PubMed).

Target Details for CBX4

Target: CBX4 (Chromobox Homolog 4 (CBX4))

Alternative Name: PC2 (CBX4) (CBX4 Products)

Synonyms: MPc2 antibody, PC2 antibody, NBP16 antibody, XPolycomb antibody, cbx4-a antibody, im:6911625 antibody, zgc:158588 antibody, chromobox 4 antibody, chromobox 4 S homeolog antibody, chromobox homolog 4 antibody, chromobox homolog 4 (Pc class homolog, Drosophila) antibody, CBX4 antibody, Cbx4 antibody, cbx4.S antibody, cbx4 antibody

Background: CBX4 is a member of Drosophila Polycomb group gene family. The polycomb group (PcG) genes are essential for maintenance of proper expression patterns of developmental master regulators, changes in expression pf PcG proteins have been associated with cancer. CBX4 is a part of the cellular memory system responsible for the inheritance of gene activity by progeny cells. It participates in maintaining the transcriptionally repressive state of genes. CBX4 is part of acomplex that acts via chromatin remodeling and modification of histones, it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. CBX4 is an E3 SUMO-protein ligase which facilitates SUMO1 conjugation by UBE2I.

Molecular Weight: 61368

Gene ID: 8535

NCBI Accession: NP_003646

UniProt: O00257