FDFT1 antibody (AA 140-170)

Catalog No. ABIN389052

Product Details anti-FDFT1 Antibody

Target: FDFT1 (Farnesyl-Diphosphate Farnesyltransferase 1 (FDFT1))

Binding Specificity: AA 140-170

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FDFT1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogen: This FDFT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 140-170 amino acids from the Central region of human FDFT1.

Clone: RB4781-4782

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:1000. WB: 1:1000. IHC-P: 1:50~100. IHC-P: 1:50~100

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

References for anti-FDFT1 antibody (ABIN389052)

Zhang, Dai, Wang: "5-Aza-2'-deoxycytidine induced growth inhibition of leukemia cells through modulating endogenous cholesterol biosynthesis." in: Molecular & cellular proteomics : MCP, Vol. 11, Issue 7, pp. M111.016915, (2012) (PubMed).

Hammer, Bien, Salazar, Steil, Scharf, Hildebrandt, Schroeder, Kroemer, Völker, Ritter: "Proteomic analysis of doxorubicin-induced changes in the proteome of HepG2cells combining 2-D DIGE and LC-MS/MS approaches." in: Proteomics, Vol. 10, Issue 1, pp. 99-114, (2010) (PubMed).

Størvold, Fleten, Olsen, Follestad, Krokan, Schønberg: "Docosahexaenoic acid activates some SREBP-2 targets independent of cholesterol and ER stress in SW620 colon cancer cells." in: Lipids, Vol. 44, Issue 8, pp. 673-83, (2009) (PubMed).

Target Details for FDFT1

Target: FDFT1 (Farnesyl-Diphosphate Farnesyltransferase 1 (FDFT1))

Alternative Name: FDFT1 (FDFT1 Products)

Synonyms: FDFT1 antibody, DKFZp459C1712 antibody, SQS antibody, SS antibody, DGPT antibody, ERG9 antibody, fdft1 antibody, farnesyl-diphosphate farnesyltransferase 1 antibody, squalene synthase antibody, farnesyl diphosphate farnesyl transferase 1 antibody, FDFT1 antibody, fdft1 antibody, LOC100560926 antibody, Fdft1 antibody

Background: FDFT1 catalyzes the first step in the cholesterol biosynthetic pathway, the conversion of trans-farnesyldiphosphate to squalene. The loss of promoter activity and response to sterols for FDFT1 is localized to a 69-bp section positioned 131 bp 5-prime to the transcription start site. Sequence analysis of this region shows that it contains a sterol regulatory element-1 (SRE1) previously identified in other sterol regulated genes and 2 putative NF1 binding sites.

Molecular Weight: 48115

Gene ID: 2222

NCBI Accession: NP_004453

UniProt: P37268

Pathways: Regulation of Lipid Metabolism by PPARalpha