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ube3a antibody (C-Term)

ube3a Reactivity: Human WB, IF, IHC (p), FACS Host: Rabbit Polyclonal RB4440 unconjugated
Catalog No. ABIN388925
  • Target See all ube3a Antibodies
    ube3a (Ubiquitin Protein Ligase E3A (ube3a))
    Binding Specificity
    • 15
    • 6
    • 5
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 836-865, C-Term
    Reactivity
    • 50
    • 11
    • 8
    • 5
    • 4
    • 4
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Host
    • 46
    • 4
    Rabbit
    Clonality
    • 46
    • 4
    Polyclonal
    Conjugate
    • 27
    • 4
    • 3
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This ube3a antibody is un-conjugated
    Application
    • 26
    • 17
    • 16
    • 13
    • 13
    • 8
    • 7
    • 7
    • 3
    • 3
    • 3
    • 2
    • 1
    Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS)
    Purification
    This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
    Immunogen
    This UBE3A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 836-865 amino acids from the C-terminal region of human UBE3A.
    Clone
    RB4440
    Isotype
    Ig Fraction
    Top Product
    Discover our top product ube3a Primary Antibody
  • Application Notes
    IF: 1:10~50. WB: 1:1000. IHC-P: 1:50~100. FC: 1:10~50
    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-20 °C
    Storage Comment
    Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
    Expiry Date
    6 months
  • Wang, Mitchell: "Cognition and Synaptic-Plasticity Related Changes in Aged Rats Supplemented with 8- and 10-Carbon Medium Chain Triglycerides." in: PLoS ONE, Vol. 11, Issue 8, pp. e0160159, (2017) (PubMed).

  • Target
    ube3a (Ubiquitin Protein Ligase E3A (ube3a))
    Alternative Name
    UBE3A (ube3a Products)
    Synonyms
    ANCR antibody, AS antibody, E6-AP antibody, EPVE6AP antibody, HPVE6A antibody, DDBDRAFT_0188760 antibody, DDBDRAFT_0302447 antibody, DDB_0188760 antibody, DDB_0302447 antibody, ube3a antibody, im:7140733 antibody, zgc:92173 antibody, 4732496B02 antibody, 5830462N02Rik antibody, A130086L21Rik antibody, Hpve6a antibody, mKIAA4216 antibody, As antibody, CG6190 antibody, Dmel\\CG6190 antibody, Dube3a antibody, UBE3A antibody, dUBE3A antibody, das antibody, dube3A antibody, dube3a antibody, MGC69536 antibody, UME3A antibody, ubiquitin protein ligase E3A antibody, ubiquitin-protein ligase E3A antibody, Ubiquitin protein ligase E3A antibody, ubiquitin protein ligase E3A (human papilloma virus E6-associated protein, Angelman syndrome) S homeolog antibody, ubiquitin protein ligase E3A (human papilloma virus E6-associated protein, Angelman syndrome) antibody, UBE3A antibody, ube3a antibody, LOC100194730 antibody, Ube3a antibody, ube3a.S antibody, Eint_040430 antibody
    Background
    UBE3A interacts with the E6 protein of the cancer-associated human papillomavirus types 16 and 18. The E6/E6-AP complex binds to and targets the p53 tumor-suppressor protein for ubiquitin-mediated proteolysis. It is an E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. It can target itself for ubiquitination in vitro and efficiently promotes its own degradation in vivo. It appears that only unmodified E6-AP molecules can bind efficiently to p53 in the presence of the HPV E6 oncoprotein. UBE3A binds UBQLN1 and UBQLN2. Defects in UBE3A are a cause of Angelman syndrome (AS) [MIM:105830], also known as 'happy puppet syndrome'. AS is characterized by features of severe motor and intellectual retardation, microcephaly, ataxia, frequent jerky limb movements and flapping of the arms and hands, hypotonia, hyperactivity, hypopigmentation, seizures, absence of speech, frequent smiling and episodes of paroxysmal laughter, and an unusual facies characterized by macrostomia, a large mandible and open-mouthed expression, a great propensity for protruding the tongue ('tongue thrusting'), and an occipital groove. UBE3A contains 1 HECT-type E3 ubiquitin-protein ligase domain.
    Molecular Weight
    100688
    Gene ID
    7337
    NCBI Accession
    NP_000453, NP_570853, NP_570854
    UniProt
    Q05086
    Pathways
    Intracellular Steroid Hormone Receptor Signaling Pathway
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