CD73 antibody (C-Term)

Catalog No. ABIN388750

Product Details anti-CD73 Antibody

Target: CD73 (NT5E) (5'-Nucleotidase, Ecto (CD73) (NT5E))

Binding Specificity: AA 520-550, C-Term

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD73 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogen: This CD73 (NT5E) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 520-550 amino acids from the C-terminal region of human CD73 (NT5E).

Clone: RB02846

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:1000. WB: 1:1000. WB: 1:500. WB: 1:1000. IHC-P: 1:10~50

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots.

Expiry Date: 6 months

References for anti-CD73 antibody (ABIN388750)

Soleimanifar, Mortazavi, Nadri, Soleimani: "Conjunctiva derived mesenchymal stem cell (CJMSCs) as a potential platform for differentiation into corneal epithelial cells on bioengineered electrospun scaffolds." in: Journal of biomedical materials research. Part A, Vol. 105, Issue 10, pp. 2703-2711, (2018) (PubMed).

Yu, Wang, Lu, Wang, Li, Liao, Zhu, Lv, Zhi, Yu, Jin, Zou, Ou, Liu, Zhou: "Extracellular 5'-nucleotidase (CD73) promotes human breast cancer cells growth through AKT/GSK-3β/β-catenin/cyclinD1 signaling pathway." in: International journal of cancer, Vol. 142, Issue 5, pp. 959-967, (2018) (PubMed).

Tak, Jung, Kim, Park, Jun, Lee, Jung, Kirchner, Hwang, Song, Lee: "Protective role of hypoxia-inducible factor-1α-dependent CD39 and CD73 in fulminant acute liver failure." in: Toxicology and applied pharmacology, Vol. 314, pp. 72-81, (2017) (PubMed).

Cartón-García, Overeem, Nieto, Bazzocco, Dopeso, Macaya, Bilic, Landolfi, Hernandez-Losa, Schwartz, Ramon Y Cajal, van Ijzendoorn, Arango: "Myo5b knockout mice as a model of microvillus inclusion disease." in: Scientific reports, Vol. 5, pp. 12312, (2015) (PubMed).

Zhi, Wang, Yu, Yu, Zhang, Yin, Zhou: "Potential prognostic biomarker CD73 regulates epidermal growth factor receptor expression in human breast cancer." in: IUBMB life, Vol. 64, Issue 11, pp. 911-20, (2012) (PubMed).

Hart, Grenz, Gorzolla, Schittenhelm, Dalton, Eltzschig: "Hypoxia-inducible factor-1?-dependent protection from intestinal ischemia/reperfusion injury involves ecto-5'-nucleotidase (CD73) and the A2B adenosine receptor." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 186, Issue 7, pp. 4367-74, (2011) (PubMed).

Zhi, Wang, Zhou, Yu, Jian, Tang, Yin, Zhou: "RNAi-mediated CD73 suppression induces apoptosis and cell-cycle arrest in human breast cancer cells." in: Cancer science, Vol. 101, Issue 12, pp. 2561-9, (2010) (PubMed).

Leth-Larsen, Lund, Hansen, Laenkholm, Tarin, Jensen, Ditzel: "Metastasis-related plasma membrane proteins of human breast cancer cells identified by comparative quantitative mass spectrometry." in: Molecular & cellular proteomics : MCP, Vol. 8, Issue 6, pp. 1436-49, (2009) (PubMed).

Hart, Henn, Köhler, Kloor, Mittelbronn, Gorzolla, Stahl, Eltzschig: "Role of extracellular nucleotide phosphohydrolysis in intestinal ischemia-reperfusion injury." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 22, Issue 8, pp. 2784-97, (2008) (PubMed).

Macdonald, Pike: "A simplified method for the preparation of detergent-free lipid rafts." in: Journal of lipid research, Vol. 46, Issue 5, pp. 1061-7, (2005) (PubMed).

Target Details for CD73

Target: CD73 (NT5E) (5'-Nucleotidase, Ecto (CD73) (NT5E))

Alternative Name: CD73 (NT5E) (NT5E Products)

Synonyms: CALJA antibody, CD73 antibody, E5NT antibody, NT antibody, NT5 antibody, NTE antibody, eN antibody, eNT antibody, zgc:63784 antibody, NT5E antibody, MGC114869 antibody, PSPTO2277 antibody, 2210401F01Rik antibody, AI447961 antibody, Nt5 antibody, 5'-nucleotidase ecto antibody, 5'-nucleotidase, ecto (CD73) antibody, 5'-nucleotidase antibody, 5' nucleotidase, ecto antibody, NT5E antibody, nt5e antibody, nt5e.S antibody, PSPTO_2277 antibody, CBU_1233 antibody, MCA1322 antibody, Nt5e antibody

Background: Ecto-5-prime-nucleotidase (5-prime-ribonucleotide phosphohydrolase) catalyzes the conversion at neutral pH of purine 5-prime mononucleotides to nucleosides, the preferred substrate being AMP. The enzyme consists of a dimer of 2 identical 70 kD subunits bound externally to the plasma membrane by a glycosyl phosphatidyl inositol linkage. The enzyme is used as a marker of lymphocyte differentiation. Consequently, a deficiency of NT5E occurs in a variety of immunodeficiency diseases. Other forms of 5-prime nucleotidase exist in the cytoplasm and lysosomes and can be distinguished from ecto-NT5 by their substrate affinities, requirement for divalent magnesium ion, activation by ATP, and inhibition by inorganic phosphate. It is not known whether the different enzymes are coded by different genes or result from different posttranslational modifications of a single coding sequence.

Molecular Weight: 63368

Gene ID: 4907

NCBI Accession: NP_001191742, NP_002517

UniProt: P21589

Pathways: Synaptic Membrane, Ribonucleoside Biosynthetic Process