PIK3C3 antibody (C-Term)

Catalog No. ABIN388591

This Rabbit Polyclonal antibody specifically detects PIK3C3 in WB and IHC (p). It exhibits reactivity toward Human and has been mentioned in 2+ publications.

Quick Overview for PIK3C3 antibody (C-Term) (ABIN388591)

Target: PIK3C3 (Phosphoinositide-3-Kinase, Class 3 (PIK3C3))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PIK3C3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: RB10857

Product Details anti-PIK3C3 Antibody

Binding Specificity: AA 770-801, C-Term

Predicted Reactivity: M, Pig, Rat

Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogen: This PI3KC3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 770-801 amino acids from the C-terminal region of human PI3KC3.

Application Details

Application Notes: WB: 1:1000. IHC-P: 1:10~50

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

References for anti-PIK3C3 antibody (ABIN388591)

Delaney, Patel, Willis, Haghighiabyaneh, Axelrod, Tancioni, Lu, Bapat, Young, Cadassou, Bartakova, Sheth, Haft, Hui, Saenz, Schlaepfer, Harismendy, Stupack: "Haploinsufficiency networks identify targetable patterns of allelic deficiency in low mutation ovarian cancer." in: Nature communications, Vol. 8, pp. 14423, (2017) (PubMed).

Liu, Ji, Zhou, Xing: "Phosphatidylinositol 3-Kinase Promotes Activation and Vacuolar Acidification and Delays Methyl Jasmonate-Induced Leaf Senescence." in: Plant physiology, Vol. 170, Issue 3, pp. 1714-31, (2016) (PubMed).

Target Details for PIK3C3

Target: PIK3C3 (Phosphoinositide-3-Kinase, Class 3 (PIK3C3))

Alternative Name: PI3KC3

Background: PI3KC3 is a catalytic subunit of the PI3K complex involved in the transport of lysosomal enzyme precursors to lysosomes. This enzyme acts catalytically to convert 1-phosphatidyl-1D-myo-inositol to 1-phosphatidyl-1D-myo-inositol 3-phosphate. Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). The regulation of the Beclin 1-PI3KC3 complex lipid kinase activity is a critical element in the autophagy signaling pathway.

Molecular Weight: 101549

Gene ID: 5289

NCBI Accession: NP_002638

UniProt: Q8NEB9

Pathways: AMPK Signaling, Activation of Innate immune Response, Inositol Metabolic Process, Toll-Like Receptors Cascades, Autophagy