Beclin 1 antibody (AA 210-239)

Catalog No. ABIN388546

Product Details anti-Beclin 1 Antibody

Target: Beclin 1 (BECN1) (Beclin 1, Autophagy Related (BECN1))

Binding Specificity: AA 210-239

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Beclin 1 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Predicted Reactivity: B, Pig, Rat

Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogen: This Beclin 1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 210-239 amino acids from human Beclin 1.

Clone: RB7463

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:4000. WB: 1:1000. WB: 1:1000. IHC-P: 1:50~100. FC: 1:10~50

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

References for anti-Beclin 1 antibody (ABIN388546)

Pla, Pascual, Guerri: "Autophagy Constitutes a Protective Mechanism against Ethanol Toxicity in Mouse Astrocytes and Neurons." in: PLoS ONE, Vol. 11, Issue 4, pp. e0153097, (2016) (PubMed).

Li, Xiong, Shi, Xiao, Qi, Meng: "IKKβ/NFκBp65 activated by interleukin-13 targets the autophagy-related genes LC3B and beclin 1 in fibroblasts co-cultured with breast cancer cells." in: Experimental and therapeutic medicine, Vol. 11, Issue 4, pp. 1259-1264, (2016) (PubMed).

Jia, Jin, Xiao, Dong, Wang, Fan, Xu, Meng, Li, Lv: "Lipoxin A4 methyl ester alleviates vascular cognition impairment by regulating the expression of proteins related to autophagy and ER stress in the rat hippocampus." in: Cellular & molecular biology letters, Vol. 20, Issue 3, pp. 475-87, (2015) (PubMed).

Liu, He, von Rütte, Yousefi, Hunger, Simon: "Down-regulation of autophagy-related protein 5 (ATG5) contributes to the pathogenesis of early-stage cutaneous melanoma." in: Science translational medicine, Vol. 5, Issue 202, pp. 202ra123, (2013) (PubMed).

Dhule, Penfornis, Frazier, Walker, Feldman, Tan, He, Alb, John, Pochampally: "Curcumin-loaded ?-cyclodextrin liposomal nanoparticles as delivery vehicles for osteosarcoma." in: Nanomedicine : nanotechnology, biology, and medicine, Vol. 8, Issue 4, pp. 440-51, (2012) (PubMed).

Wang, Whiteman, Lian, Wang, Singh, Huang, Denmark: "A non-canonical MEK/ERK signaling pathway regulates autophagy via regulating Beclin 1." in: The Journal of biological chemistry, Vol. 284, Issue 32, pp. 21412-24, (2009) (PubMed).

Target Details for Beclin 1

Target: Beclin 1 (BECN1) (Beclin 1, Autophagy Related (BECN1))

Alternative Name: Beclin 1 (BECN1 Products)

Synonyms: beclin-1 antibody, atg6 antibody, vps30 antibody, beclin1 antibody, Beclin-1 antibody, becn1 antibody, zgc:73097 antibody, ATG6 antibody, VPS30 antibody, 4921513J16Rik antibody, 5430417M23Rik antibody, Atg6 antibody, beclin 1 antibody, beclin 1, autophagy related antibody, beclin 1 protein antibody, beclin-1 antibody, beclin family protein antibody, beclin 1, autophagy related L homeolog antibody, BECN1 antibody, becn1 antibody, atg6 antibody, CpipJ_CPIJ011087 antibody, PTRG_07825 antibody, SJAG_01876 antibody, Becn1 antibody, becn1.L antibody

Background: Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). Beclin 1 plays a role in two fundamentally important cell biological pathways: autophagy and apoptosis. Beclin 1 is thought to function as a VPS and autophagy protein as part of a complex with Class III PI3 kinase, Vps34.

Molecular Weight: 51896

Gene ID: 8678

NCBI Accession: NP_003757

UniProt: Q14457

Pathways: Autophagy