ATG16L1 antibody (C-Term)

Catalog No. ABIN388543

Product Details anti-ATG16L1 Antibody

Target: ATG16L1 (ATG16 Autophagy Related 16-Like 1 (ATG16L1))

Binding Specificity: AA 454-483, C-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATG16L1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogen: This ATG16L antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 454-483 amino acids from the C-terminal region of human ATG16L.

Clone: RB7491

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:1000. IHC-P: 1:50~100

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

References for anti-ATG16L1 antibody (ABIN388543)

Gao, Kang, Liao, Ding, Gambotto, Watkins, Liu, Stolz, Yin: "Biochemical isolation and characterization of the tubulovesicular LC3-positive autophagosomal compartment." in: The Journal of biological chemistry, Vol. 285, Issue 2, pp. 1371-83, (2010) (PubMed).

Target Details for ATG16L1

Target: ATG16L1 (ATG16 Autophagy Related 16-Like 1 (ATG16L1))

Alternative Name: ATG16L (ATG16L1 Products)

Synonyms: APG16L antibody, ATG16A antibody, ATG16L antibody, IBD10 antibody, WDR30 antibody, 1500009K01Rik antibody, Apg16l antibody, Atg16l antibody, Wdr30 antibody, atg16 antibody, atg16l antibody, fb57e05 antibody, wu:fb57e05 antibody, zgc:110147 antibody, autophagy related 16 like 1 antibody, autophagy related 16-like 1 (S. cerevisiae) antibody, autophagy related 16-like 1 antibody, autophagy related 16 like 1 S homeolog antibody, ATG16 autophagy related 16-like 1 (S. cerevisiae) antibody, ATG16L1 antibody, Atg16l1 antibody, atg16l1 antibody, atg16l1.S antibody

Background: Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). The APG12-APG5-APG16L complex is esential for the elongation of autophagic isolation membranes. This complex initially associates in uniform distribution with small vesicle membranes. During membrane elongation, the complex partitions, with a great concentration building on the outer side of the isolation membrane. Upon completion of the formation of the autophagosome, the APG12-APG5-APG16L dissociates from the membrane.

Molecular Weight: 68265

Gene ID: 55054

NCBI Accession: NP_001177195, NP_001177196, NP_060444, NP_110430, NP_942593

UniProt: Q676U5

Pathways: Autophagy