ATG12 antibody (N-Term)

Catalog No. ABIN388536

Product Details anti-ATG12 Antibody

Target: ATG12 (Autophagy Related 12 (ATG12))

Binding Specificity: AA 1-30, N-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATG12 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This ATG12 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human ATG12.

Clone: RB7475

Isotype: Ig Fraction

Application Details

Application Notes: IF: 1:200. WB: 1:2000. IHC-P: 1:50~100. IHC-P: 1:50~100

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

References for anti-ATG12 antibody (ABIN388536)

Yang, Zhao, Mei, Jiang, Geng, Li, Yao, Liu, Kong, Cao: "HMGA2 plays an important role in Cr (VI)-induced autophagy." in: International journal of cancer, Vol. 141, Issue 5, pp. 986-997, (2017) (PubMed).

Chen, Cao, Zhou, Liu, Che, Mizumura, Li, Choi, Shen: "Interaction of caveolin-1 with ATG12-ATG5 system suppresses autophagy in lung epithelial cells." in: American journal of physiology. Lung cellular and molecular physiology, Vol. 306, Issue 11, pp. L1016-25, (2014) (PubMed).

Sanchez, Penfornis, Oskowitz, Boonjindasup, Cai, Dhule, Rowan, Kelekar, Krause, Pochampally: "Activation of autophagy in mesenchymal stem cells provides tumor stromal support." in: Carcinogenesis, Vol. 32, Issue 7, pp. 964-72, (2011) (PubMed).

Yee, Wilkinson, James, Ryan, Vousden: "PUMA- and Bax-induced autophagy contributes to apoptosis." in: Cell death and differentiation, Vol. 16, Issue 8, pp. 1135-45, (2009) (PubMed).

Harada, Willison, Sakakibara, Miyamoto, Fujita, Taniguchi: "Absence of the type I IFN system in EC cells: transcriptional activator (IRF-1) and repressor (IRF-2) genes are developmentally regulated." in: Cell, Vol. 63, Issue 2, pp. 303-12, (1990) (PubMed).

Target Details for ATG12

Target: ATG12 (Autophagy Related 12 (ATG12))

Alternative Name: ATG12 (ATG12 Products)

Background: Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). APG12L is the human homolog of yeast APG12, a ubiquitin-activating enzyme E1-like protein essential for the conjugation system that mediates membrane fusion in autophagy.

Molecular Weight: 15113

Gene ID: 9140

NCBI Accession: NP_001264712, NP_004698

UniProt: O94817

Pathways: Autophagy