ATG7 antibody (C-Term)

Catalog No. ABIN388523

Product Details anti-ATG7 Antibody

Target: ATG7 (ATG7 Autophagy Related 7 (ATG7))

Binding Specificity: AA 540-569, C-Term

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATG7 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Predicted Reactivity: C, Rat

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This ATG7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 540-569 amino acids from the C-terminal region of human ATG7.

Clone: RB7474

Isotype: Ig Fraction

Application Details

Application Notes: IF: 1:25. WB: 1:1000. WB: 1:1000. WB: 1:500. IHC-P-Leica: 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

References for anti-ATG7 antibody (ABIN388523)

Wu, Sun, Ma, Zhu, Zhang, Pan, Li, Liu, Diao, Li: "A C-terminally truncated mouse Best3 splice variant targets and alters the ion balance in lysosome-endosome hybrids and the endoplasmic reticulum." in: Scientific reports, Vol. 6, pp. 27332, (2018) (PubMed).

Ding, Shi, Zhou, Qiu, Xu, Dai, Shi, Wang, Ke, Wu, Fan: "Association of autophagy defect with a malignant phenotype and poor prognosis of hepatocellular carcinoma." in: Cancer research, Vol. 68, Issue 22, pp. 9167-75, (2008) (PubMed).

Target Details for ATG7

Target: ATG7 (ATG7 Autophagy Related 7 (ATG7))

Alternative Name: ATG7 (ATG7 Products)

Synonyms: APG7-LIKE antibody, APG7L antibody, GSA7 antibody, D8 antibody, UBA1B antibody, UBE1L antibody, UBE2 antibody, Ube1l antibody, 1300004C08Rik antibody, AO090001000744 antibody, atg7 antibody, 1810013K23Rik antibody, Agp7 antibody, Apg7l antibody, Atg7l antibody, ATAPG7 antibody, ATATG7 antibody, ATG7 antibody, AUTOPHAGY 7 antibody, AUTOPHAGY-RELATED 7 antibody, K15I22.10 antibody, K15I22_10 antibody, autophagy related 7 antibody, ubiquitin like modifier activating enzyme 7 antibody, ubiquitin-like modifier activating enzyme 7 antibody, autophagy-related protein 7 antibody, Autophagy-related protein 7 antibody, Apg7p antibody, ThiF family protein antibody, ubiquitin-like conjugating enzyme Atg7 antibody, ATG7 antibody, UBA7 antibody, Uba7 antibody, ANI_1_3368024 antibody, AOR_1_1338164 antibody, PTRG_02236 antibody, PAAG_00287 antibody, PITG_12537 antibody, atg7 antibody, Atg7 antibody, APG7 antibody

Background: Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). APG7 functions as an E1 enzyme essential for multisubstrates such as GABARAPL1 and ATG12. APG3L is an E2-like conjugating enzyme facilitating covalent binding of APG8 (MAP1LC3) to phosphatidylethanolamine (PE). APG7 (an E1-like enzyme) facilitates this reaction by forming an E1-E2 complex with APG3. Formation of the PE conjugate is essential for autophagy.

Molecular Weight: 77960

Gene ID: 10533

NCBI Accession: NP_001129503, NP_001138384, NP_006386

UniProt: O95352

Pathways: Response to Water Deprivation, Autophagy