ATG5 antibody (N-Term)

Catalog No. ABIN388518

Product Details anti-ATG5 Antibody

Target: ATG5 (ATG5 Autophagy Related 5 (ATG5))

Binding Specificity: AA 1-30, N-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATG5 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Predicted Reactivity: Zf, B, M, Pig, Rat

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This ATG5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human ATG5.

Clone: RB7466

Isotype: Ig Fraction

Application Details

Application Notes: IF: 1:200. WB: 1:1000. IHC-P-Leica: 1:500

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

References for anti-ATG5 antibody (ABIN388518)

Kessel, Reiners: "Effects of Combined Lysosomal and Mitochondrial Photodamage in a Non-small-Cell Lung Cancer Cell Line: The Role of Paraptosis." in: Photochemistry and photobiology, Vol. 93, Issue 6, pp. 1502-1508, (2018) (PubMed).

Kessel, Evans: "Promotion of Proapoptotic Signals by Lysosomal Photodamage: Mechanistic Aspects and Influence of Autophagy." in: Photochemistry and photobiology, Vol. 92, Issue 4, pp. 620-3, (2017) (PubMed).

Mathai, Meijer, Simonsen: "Studying Autophagy in Zebrafish." in: Cells, Vol. 6, Issue 3, (2017) (PubMed).

Lim, Zare, Puertollano, Raben: "Atg5flox-Derived Autophagy-Deficient Model of Pompe Disease: Does It Tell the Whole Story?" in: Molecular therapy. Methods & clinical development, Vol. 7, pp. 11-14, (2017) (PubMed).

Sasaki, Yamashita, Shin: "Autophagy in spinal motor neurons of conditional ADAR2-knockout mice: An implication for a role of calcium in increased autophagy flux in ALS." in: Neuroscience letters, Vol. 598, pp. 79-84, (2015) (PubMed).

Wang, Qi, Wang, Zhu, Wang, Jin, Tan, Huang, Xu, Li, Kuang, Tang, Du, Chen, Chen: "FGFR3/Fibroblast Growth Factor Receptor 3 Inhibits Autophagy through Decreasing the ATG12-ATG5 Conjugate, Leading to the Delay of Cartilage Development in Achondroplasia." in: Autophagy, (2015) (PubMed).

Chen, Huang, Yuan, Cheng, Zhou: "Long-term artificial selection reveals a role of TCTP in autophagy in mammalian cells." in: Molecular biology and evolution, Vol. 31, Issue 8, pp. 2194-211, (2014) (PubMed).

Xin, Xiao, Ma, He, Yao, Liu et al.: "Coxsackievirus B3 induces crosstalk between autophagy and apoptosis to benefit its release after replicating in autophagosomes through a mechanism involving caspase cleavage of autophagy-related ..." in: Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases, Vol. 26, pp. 95-102, (2014) (PubMed).

Cha, Hwang, Kim, Choi, Oh, Roh: "Autophagy induced by tumor necrosis factor ? mediates intrinsic apoptosis in trophoblastic cells." in: Reproductive sciences (Thousand Oaks, Calif.), Vol. 21, Issue 5, pp. 612-22, (2014) (PubMed).

Chinskey, Zheng, Zacks: "Control of photoreceptor autophagy after retinal detachment: the switch from survival to death." in: Investigative ophthalmology & visual science, Vol. 55, Issue 2, pp. 688-95, (2014) (PubMed).

Schober, Parlato, Huber, Kinscherf, Hartleben, Huber, Schütz, Unsicker: "Cell loss and autophagy in the extra-adrenal chromaffin organ of Zuckerkandl are regulated by glucocorticoid signalling." in: Journal of neuroendocrinology, Vol. 25, Issue 1, pp. 34-47, (2013) (PubMed).

Keil, Höcker, Schuster, Essmann, Ueffing, Hoffman, Liebermann, Pfeffer, Schulze-Osthoff, Schmitz: "Phosphorylation of Atg5 by the Gadd45?-MEKK4-p38 pathway inhibits autophagy." in: Cell death and differentiation, Vol. 20, Issue 2, pp. 321-32, (2013) (PubMed).

Khaldoun, Emond-Boisjoly, Chateau, Carrière, Lacasa, Rousset, Demignot, Morel: "Autophagosomes contribute to intracellular lipid distribution in enterocytes." in: Molecular biology of the cell, Vol. 25, Issue 1, pp. 118-32, (2013) (PubMed).

Liu, He, von Rütte, Yousefi, Hunger, Simon: "Down-regulation of autophagy-related protein 5 (ATG5) contributes to the pathogenesis of early-stage cutaneous melanoma." in: Science translational medicine, Vol. 5, Issue 202, pp. 202ra123, (2013) (PubMed).

Chiu, Chen, Ho, Wang: "Arsenic trioxide enhances the radiation sensitivity of androgen-dependent and -independent human prostate cancer cells." in: PLoS ONE, Vol. 7, Issue 2, pp. e31579, (2012) (PubMed).

Hu, Zhang, Zhang: "Expression pattern and functions of autophagy-related gene atg5 in zebrafish organogenesis." in: Autophagy, Vol. 7, Issue 12, pp. 1514-27, (2012) (PubMed).

Lépine, Allegood, Edmonds, Milstien, Spiegel: "Autophagy induced by deficiency of sphingosine-1-phosphate phosphohydrolase 1 is switched to apoptosis by calpain-mediated autophagy-related gene 5 (Atg5) cleavage." in: The Journal of biological chemistry, Vol. 286, Issue 52, pp. 44380-90, (2011) (PubMed).

Chiu, Ho, Wang: "Arsenic trioxide induces autophagy and apoptosis in human glioma cells in vitro and in vivo through downregulation of survivin." in: Journal of molecular medicine (Berlin, Germany), Vol. 89, Issue 9, pp. 927-41, (2011) (PubMed).

Lépine, Allegood, Park, Dent, Milstien, Spiegel: "Sphingosine-1-phosphate phosphohydrolase-1 regulates ER stress-induced autophagy." in: Cell death and differentiation, Vol. 18, Issue 2, pp. 350-61, (2011) (PubMed).

Hnasko, Lin, McGarvey, Stanker: "A rapid method to improve protein detection by indirect ELISA." in: Biochemical and biophysical research communications, Vol. 410, Issue 4, pp. 726-31, (2011) (PubMed).

Target Details for ATG5

Target: ATG5 (ATG5 Autophagy Related 5 (ATG5))

Alternative Name: ATG5 (ATG5 Products)

Synonyms: APG5 antibody, APG5-LIKE antibody, APG5L antibody, ASP antibody, hAPG5 antibody, ATG5 antibody, CG1643 antibody, DmAtg5 antibody, Dmel\\CG1643 antibody, atg5 antibody, 2010107M05Rik antibody, 3110067M24Rik antibody, AW319544 antibody, Apg5l antibody, Atg5l antibody, C88337 antibody, Paddy antibody, apg5l antibody, zgc:100934 antibody, ATATG5 antibody, AUTOPHAGY 5 antibody, MKP11.20 antibody, MKP11_20 antibody, autophagy related 5 antibody, Autophagy-related 5 antibody, ATG5 autophagy related 5 homolog (S. cerevisiae) antibody, autophagy related 5 L homeolog antibody, similar to S. cerevisiae ATG5 (YPL149W) which nucleates preautophagosome formation as a conjugate with Atg12 antibody, autophagy protein Apg5 family antibody, ATG5 antibody, Atg5 antibody, atg5 antibody, atg5.L antibody, APG5 antibody

Background: Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). APG5, required for autophagy, conjugates to ATG12 and associates with an isolation membrane to form a cup-shaped isolation membrane and autophagosome. The conjugate detaches from the membrane immediately before or after autophagosome formation is completed. APG5 may also play an important role in the apoptotic process, possibly within the modified cytoskeleton. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity.

Molecular Weight: 32447

Gene ID: 9474

NCBI Accession: NP_004840

UniProt: Q9H1Y0

Pathways: Activation of Innate immune Response, Production of Molecular Mediator of Immune Response, Autophagy