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PUMA antibody (AA 119-154)

Name: PUMA antibody (AA 119-154)
SKU: ABIN388112
Price: 601 USD
Availability: InStock

BBC3 Reactivity: Human WB, FACS, IHC (p), IF Host: Rabbit Polyclonal RB01353 unconjugated

Western blot analysis of anti-Puma BH3 domain Pab (ABIN388112 and ABIN2846254) in HL-60 cell lysate. (PUMA antibody (AA 119-154))

Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with Puma BH3 Domain antibody (ABIN388112 and ABIN2846254) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. (PUMA antibody (AA 119-154))

IHC (p)

Confocal immunofluorescent analysis of Puma BH3 Domain Antibody (ABIN388112 and ABIN2846254) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). (PUMA antibody (AA 119-154))

Puma BH3 Domain Antibody (ABIN388112 and ABIN2846254) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram). (PUMA antibody (AA 119-154))

FACS

4 images

(1 reference)

Catalog No. ABIN388112

Datasheet as PDF

Target See all PUMA (BBC3) Antibodies

PUMA (BBC3) (BCL2 Binding Component 3 (BBC3))
Binding Specificity
16

13

9

7

7

6

6

6

5

5

4

2

1

1

1

1

1

1

1

1
AA 119-154
Reactivity
92

40

38

16
Human
Host
89

6

1
Rabbit
Compatible Secondaries
Clonality
80

16
Polyclonal
Conjugate
48

6

5

5

3

3

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1
This PUMA antibody is un-conjugated
Application
77

34

29

21

16

14

13

12

9

8

5

3

2

2
Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Predicted Reactivity

M, Rat

Purification

This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogen

This Puma BH3 Domain antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 119-154 amino acids from human Puma BH3 Domain.

Clone

RB01353

Isotype

Ig Fraction

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anti-BCL2 Binding Component 3 (BBC3) (C-Term) antibody
BBC3 Reactivity: Human WB, ICC, IF Host: Rabbit Polyclonal unconjugated

anti-BCL2 Binding Component 3 (BBC3) (AA 131-180) antibody
BBC3 Reactivity: Human, Rat, Pig WB, ELISA, IP, FACS, IHC (p), IF (cc), IF (p), IHC (fro) Host: Rabbit Polyclonal unconjugated

anti-BCL2 Binding Component 3 (BBC3) (AA 119-154) antibody
BBC3 Reactivity: Human WB, ELISA, IHC, FACS, IF Host: Rabbit Polyclonal unconjugated

anti-BCL2 Binding Component 3 (BBC3) (N-Term) antibody
BBC3 Reactivity: Human WB, IHC Host: Rabbit Polyclonal unconjugated

anti-BCL2 Binding Component 3 (BBC3) (C-Term) antibody (PE)
BBC3 Reactivity: Human WB, ICC, IF Host: Rabbit Polyclonal PE

anti-BCL2 Binding Component 3 (BBC3) (C-Term) antibody (PerCP)
BBC3 Reactivity: Human WB, ICC, IF Host: Rabbit Polyclonal PerCP

anti-BCL2 Binding Component 3 (BBC3) (N-Term) antibody (Biotin)
BBC3 Reactivity: Human WB, IHC Host: Rabbit Polyclonal Biotin

anti-BCL2 Binding Component 3 (BBC3) (BH3 Domain), (C-Term) antibody
BBC3 Reactivity: Human, Mouse WB, FACS, IHC (p), IF, EIA Host: Rabbit Polyclonal unconjugated

anti-BCL2 Binding Component 3 (BBC3) (AA 109-137) antibody
BBC3 Reactivity: Human WB, IHC (p) Host: Rabbit Polyclonal RB33714 unconjugated

anti-BCL2 Binding Component 3 (BBC3) (C-Term) antibody
BBC3 Reactivity: Human, Mouse WB, IHC, ICC, IHC (p) Host: Rabbit Polyclonal unconjugated

Application Notes

IF: 1:10~50. WB: 1:1000. IHC-P: 1:10~50. FC: 1:10~50

Restrictions

For Research Use only
Format

Liquid

Buffer

Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

4 °C,-20 °C

Storage Comment

Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date

6 months
Vesela, Matalova: "Detection of Bim and Puma in mouse hair follicles using immunofluorescence and TUNEL assay double staining." in: Biotechnic & histochemistry : official publication of the Biological Stain Commission, pp. 1-7, (2015) (PubMed).
Target

PUMA (BBC3) (BCL2 Binding Component 3 (BBC3))

Alternative Name

Puma (BBC3 Products)

Synonyms

BBC3 antibody, Puma antibody, JFY-1 antibody, JFY1 antibody, PUMA antibody, PUMA/JFY1 antibody, BCL2 binding component 3 antibody, Bcl-2 binding component 3 antibody, BBC3 antibody, Bbc3 antibody, bbc3 antibody

Background

PUMA is one of the pro-apoptotic Bcl-2 family members including Bax and Noxa, which are also transcriptional targets of p53. The PUMA gene encodes two BH3 domain-containing proteins termed PUMA-a and PUMA-b. PUMA proteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53. PUMA may be a direct mediator of p53-induced apoptosis.

Molecular Weight

20532

Gene ID

27113

NCBI Accession

NP_001120712, NP_001120713, NP_001120714, NP_055232

UniProt

Q9BXH1

Pathways

p53 Signaling, Positive Regulation of Endopeptidase Activity