Bcl-2 antibody (AA 75-110)

Catalog No. ABIN388095

Product Details anti-Bcl-2 Antibody

Target: Bcl-2 (BCL2) (B-Cell CLL/lymphoma 2 (BCL2))

Binding Specificity: AA 75-110

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Bcl-2 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Predicted Reactivity: B

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This Bcl antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 75-110 amino acids from human Bcl.

Clone: RB04684

Isotype: Ig Fraction

Application Details

Application Notes: IF: 1:10~50. WB: 1:1000. IHC-P: 1:100. IHC-P: 1:100

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

References for anti-Bcl-2 antibody (ABIN388095)

Wang, Kong, Lin, Li, Izumiya, Ding, Zhang, Hu, Yang, Gao, Lam, Li: "A versatile nanoplatform for synergistic combination therapy to treat human esophageal cancer." in: Acta pharmacologica Sinica, Vol. 38, Issue 6, pp. 931-942, (2018) (PubMed).

Cui, Lin, Shi, Pan: "Hypoxic postconditioning attenuates apoptosis via inactivation of adenosine A2a receptor through NDRG3-Raf-ERK pathway." in: Biochemical and biophysical research communications, Vol. 491, Issue 2, pp. 277-284, (2017) (PubMed).

Zheng, Xu, Sun, Yin, Dong, Zeng: "Synergism between thioredoxin reductase inhibitor ethaselen and sodium selenite in inhibiting proliferation and inducing death of human non-small cell lung cancer cells." in: Chemico-biological interactions, Vol. 275, pp. 74-85, (2017) (PubMed).

Hu, Kuo, Senadi, Chang, Wang: "Photodynamic Therapy Using Indolines-Fused-Triazoles Induces Mitochondrial Apoptosis in Human Non-Melanoma BCC Cells." in: Anticancer research, Vol. 37, Issue 10, pp. 5499-5505, (2017) (PubMed).

Kiss, Tóth, Sarang, Garabuczi, Szondy: "Retinoids induce Nur77-dependent apoptosis in mouse thymocytes." in: Biochimica et biophysica acta, Vol. 1853, Issue 3, pp. 660-70, (2015) (PubMed).

Hu, Baldwin: "Differential roles for Bim and Nur77 in thymocyte clonal deletion induced by ubiquitous self-antigen." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 194, Issue 6, pp. 2643-53, (2015) (PubMed).

Cai, Xia, Yang, Liu, Shao, Liu, Yang, Xiong: "Ropivacaine- and bupivacaine-induced death of rabbit annulus fibrosus cells in vitro: involvement of the mitochondrial apoptotic pathway." in: Osteoarthritis and cartilage / OARS, Osteoarthritis Research Society, (2015) (PubMed).

Li, Wang, Wang, Gao, Zhang, Lu: "Oridonin phosphate-induced autophagy effectively enhances cell apoptosis of human breast cancer cells." in: Medical oncology (Northwood, London, England), Vol. 32, Issue 1, pp. 365, (2014) (PubMed).

Pickles, Destroismaisons, Peyrard, Cadot, Rouleau, Brown, Julien, Arbour, Vande Velde: "Mitochondrial damage revealed by immunoselection for ALS-linked misfolded SOD1." in: Human molecular genetics, Vol. 22, Issue 19, pp. 3947-59, (2013) (PubMed).

Chiavarina, Whitaker-Menezes, Martinez-Outschoorn, Witkiewicz, Birbe, Howell, Pestell, Smith, Daniel, Sotgia, Lisanti: "Pyruvate kinase expression (PKM1 and PKM2) in cancer-associated fibroblasts drives stromal nutrient production and tumor growth." in: Cancer biology & therapy, Vol. 12, Issue 12, pp. 1101-13, (2012) (PubMed).

Zhao, He, Liu, Zhu, Tian, Gao, He, Yue, Lei, Ni, Wang, Jin, Hao, Lin, Chen: "Natural diterpenoid compound elevates expression of Bim protein, which interacts with antiapoptotic protein Bcl-2, converting it to proapoptotic Bax-like molecule." in: The Journal of biological chemistry, Vol. 287, Issue 2, pp. 1054-65, (2012) (PubMed).

Guareschi, Cova, Cereda, Ceroni, Donetti, Bosco, Trotti, Pasinelli: "An over-oxidized form of superoxide dismutase found in sporadic amyotrophic lateral sclerosis with bulbar onset shares a toxic mechanism with mutant SOD1." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 109, Issue 13, pp. 5074-9, (2012) (PubMed).

Zheng, Zhou, Ye, Li, Bai, Zhang: "The conformation change of Bcl-2 is involved in arsenic trioxide-induced apoptosis and inhibition of proliferation in SGC7901 human gastric cancer cells." in: World journal of surgical oncology, Vol. 8, Issue 1, pp. 31, (2010) (PubMed).

Pedrini, Sau, Guareschi, Bogush, Brown, Naniche, Kia, Trotti, Pasinelli: "ALS-linked mutant SOD1 damages mitochondria by promoting conformational changes in Bcl-2." in: Human molecular genetics, Vol. 19, Issue 15, pp. 2974-86, (2010) (PubMed).

Ferlini, Cicchillitti, Raspaglio, Bartollino, Cimitan, Bertucci, Mozzetti, Gallo, Persico, Fattorusso, Campiani, Scambia: "Paclitaxel directly binds to Bcl-2 and functionally mimics activity of Nur77." in: Cancer research, Vol. 69, Issue 17, pp. 6906-14, (2009) (PubMed).

Deng, Gao, May: "Protein phosphatase 2A inactivates Bcl2's antiapoptotic function by dephosphorylation and up-regulation of Bcl2-p53 binding." in: Blood, Vol. 113, Issue 2, pp. 422-8, (2009) (PubMed).

Kolluri, Zhu, Zhou, Lin, Chen, Sun, Tian, Town, Cao, Lin, Zhai, Kitada, Luciano, ODonnell, Cao, He, Lin, Reed, Satterthwait, Zhang: "A short Nur77-derived peptide converts Bcl-2 from a protector to a killer." in: Cancer cell, Vol. 14, Issue 4, pp. 285-98, (2008) (PubMed).

Thompson, Winoto: "During negative selection, Nur77 family proteins translocate to mitochondria where they associate with Bcl-2 and expose its proapoptotic BH3 domain." in: The Journal of experimental medicine, Vol. 205, Issue 5, pp. 1029-36, (2008) (PubMed).

Del Gaizo Moore, Schlis, Sallan, Armstrong, Letai: "BCL-2 dependence and ABT-737 sensitivity in acute lymphoblastic leukemia." in: Blood, Vol. 111, Issue 4, pp. 2300-9, (2008) (PubMed).

Liu, Zhou, Li, Sun, Lin, Lang, He, Cao, Yan, Wang, Lu, Han, Cao, Zhang, Zeng: "Modulation of orphan nuclear receptor Nur77-mediated apoptotic pathway by acetylshikonin and analogues." in: Cancer research, Vol. 68, Issue 21, pp. 8871-80, (2008) (PubMed).

Target Details for Bcl-2

Target: Bcl-2 (BCL2) (B-Cell CLL/lymphoma 2 (BCL2))

Alternative Name: Bcl-2 (BCL2 Products)

Synonyms: Bcl-2 antibody, PPP1R50 antibody, bcl-2 antibody, AW986256 antibody, C430015F12Rik antibody, D630044D05Rik antibody, D830018M01Rik antibody, BCL-2 antibody, Bcl2 antibody, BCL2, apoptosis regulator antibody, B cell leukemia/lymphoma 2 antibody, B-cell CLL/lymphoma 2a antibody, B-cell CLL/lymphoma 2 protein antibody, BCL2 antibody, Bcl2 antibody, bcl2a antibody, BCL-2 antibody

Background: BCL2 is integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma.

Molecular Weight: 26266

Gene ID: 596

NCBI Accession: NP_000624

UniProt: P10415

Pathways: MAPK Signaling, PI3K-Akt Signaling, Apoptosis, Caspase Cascade in Apoptosis, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Skeletal Muscle Fiber Development, Autophagy, Smooth Muscle Cell Migration, Negative Regulation of intrinsic apoptotic Signaling