SET7 antibody (C-Term)

Catalog No. ABIN387998

Product Details anti-SET7 Antibody

Target: SET7 (SETD8P1) (SET Domain Containing 8 Pseudogene 1 (SETD8P1))

Binding Specificity: AA 289-317, C-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SET7 antibody is un-conjugated

Application: Western Blotting (WB)

Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogen: This SET7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 289-317 amino acids from the C-terminal region of human SET7.

Clone: RB5744

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

Target Details for SET7

Target: SET7 (SETD8P1) (SET Domain Containing 8 Pseudogene 1 (SETD8P1))

Alternative Name: SET7 (SETD8P1 Products)

Synonyms: KMT7 antibody, SET7 antibody, SET7/9 antibody, SET9 antibody, 1600028F23Rik antibody, H3K4MT antibody, Set7 antibody, Set7/9 antibody, mKIAA1717 antibody, SET domain containing lysine methyltransferase 7 antibody, SET domain containing (lysine methyltransferase) 7 antibody, SETD7 antibody, Setd7 antibody

Background: Histone methyltransferases (HMTases) selectively methylate evolutionarily conserved arginine or lysine residues, primarily in the N-terminal tails of histones H3 and H4. Signal transduction pathways affecting the N-terminal tails of histones lead to a number of post-translational modifications including acetylation, phosphorylation, poly(ADP-ribosylation), ubiquitination and methylation. These modifications play critical roles in regulating chromatin structure and gene expression. Set7/9 is a histone specific HMTase that methylates histone H3 lysine 4. Set7/9 transfers methyl groups to lysine 4 of histone H3 in complex with S-adenosyl-L-methionine. In yeast, H4-K20 methylation does not have any apparent role in the regulation of gene expression or heterochromatin function, rather it appears to play a role in DNA damage response. Loss of Set9 activity or mutation of H4-K20 markedly impairs yeast cell survival after genotoxic challenge and compromises the ability of cells to maintain checkpoint mediated cell cycle arrest. Genetic experiments link Set9 to Crb2, a homolog of the mammalian checkpoint protein 53BP1, and the enzyme is required for Crb2 localization to sites of DNA damage.

Molecular Weight: 40721

Gene ID: 80854

NCBI Accession: NP_085151

UniProt: Q8WTS6