Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Predicted Reactivity
M, Rat
Purification
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Immunogen
This Dnmt3a antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 31-61 amino acids from the N-terminal region of human Dnmt3a.
CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. Dnmt3a is a DNA methyltransferase that is thought to function in de novo methylation, rather than maintenance methylation. The protein localizes to the cytoplasm and nucleus and its expression is developmentally regulated.