DNMT3A antibody (AA 457-486)

Catalog No. ABIN387881

Product Details anti-DNMT3A Antibody

Target: DNMT3A (DNA (Cytosine-5-)-Methyltransferase 3 alpha (DNMT3A))

Binding Specificity: AA 457-486

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DNMT3A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Predicted Reactivity: C

Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogen: This Dnmt3a antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 457-486 amino acids from human Dnmt3a.

Clone: RB01852

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:1000. WB: 1:1000. WB: 1:1000. WB: 1:1000. IHC-P: 1:50~100

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

References for anti-DNMT3A antibody (ABIN387881)

Bing, Zhu, Yu, Li, Liu, Li, Wang, Qi, Guo, Yuan, He, Liu, Liu: "Glucocorticoid-induced S-adenosylmethionine enhances the interferon signaling pathway by restoring STAT1 protein methylation in hepatitis B virus-infected cells." in: The Journal of biological chemistry, Vol. 289, Issue 47, pp. 32639-55, (2014) (PubMed).

Siddiqi, Terry, Matushansky: "Hiwi mediated tumorigenesis is associated with DNA hypermethylation." in: PLoS ONE, Vol. 7, Issue 3, pp. e33711, (2012) (PubMed).

Koturbash, Zemp, Kolb, Kovalchuk: "Sex-specific radiation-induced microRNAome responses in the hippocampus, cerebellum and frontal cortex in a mouse model." in: Mutation research, Vol. 722, Issue 2, pp. 114-8, (2011) (PubMed).

Chen, Wang, Hu, Chang, Liao, Dai, Juo: "OxLDL up-regulates microRNA-29b, leading to epigenetic modifications of MMP-2/MMP-9 genes: a novel mechanism for cardiovascular diseases." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 25, Issue 5, pp. 1718-28, (2011) (PubMed).

Yamagata, Asada, Tamura, Lee, Maekawa, Taniguchi, Taketani, Matsuoka, Tamura, Sugino: "DNA methyltransferase expression in the human endometrium: down-regulation by progesterone and estrogen." in: Human reproduction (Oxford, England), Vol. 24, Issue 5, pp. 1126-32, (2009) (PubMed).

Ishii, Kohu, Yamada, Ishidoya, Kanto, Fuji, Moriya, Satake, Arai: "Up-regulation of DNA-methyltransferase 3A expression is associated with hypomethylation of intron 25 in human testicular germ cell tumors." in: The Tohoku journal of experimental medicine, Vol. 212, Issue 2, pp. 177-90, (2007) (PubMed).

Koturbash, Boyko, Rodriguez-Juarez, McDonald, Tryndyak, Kovalchuk, Pogribny, Kovalchuk: "Role of epigenetic effectors in maintenance of the long-term persistent bystander effect in spleen in vivo." in: Carcinogenesis, Vol. 28, Issue 8, pp. 1831-8, (2007) (PubMed).

Tryndyak, Muskhelishvili, Kovalchuk, Rodriguez-Juarez, Montgomery, Churchwell, Ross, Beland, Pogribny: "Effect of long-term tamoxifen exposure on genotoxic and epigenetic changes in rat liver: implications for tamoxifen-induced hepatocarcinogenesis." in: Carcinogenesis, Vol. 27, Issue 8, pp. 1713-20, (2006) (PubMed).

Koturbash, Rugo, Hendricks, Loree, Thibault, Kutanzi, Pogribny, Yanch, Engelward, Kovalchuk: "Irradiation induces DNA damage and modulates epigenetic effectors in distant bystander tissue in vivo." in: Oncogene, Vol. 25, Issue 31, pp. 4267-75, (2006) (PubMed).

Pogribny, Koturbash, Tryndyak, Hudson, Stevenson, Sedelnikova, Bonner, Kovalchuk: "Fractionated low-dose radiation exposure leads to accumulation of DNA damage and profound alterations in DNA and histone methylation in the murine thymus." in: Molecular cancer research : MCR, Vol. 3, Issue 10, pp. 553-61, (2005) (PubMed).

Target Details for DNMT3A

Target: DNMT3A (DNA (Cytosine-5-)-Methyltransferase 3 alpha (DNMT3A))

Alternative Name: Dnmt3a (DNMT3A Products)

Synonyms: DNMT3A antibody, DNMT3A2 antibody, M.HsaIIIA antibody, MmuIIIA antibody, dnmt3a1 antibody, dnmt6 antibody, DNA methyltransferase 3 alpha antibody, DNA methyltransferase 3A antibody, DNA (cytosine-5-)-methyltransferase 3 alpha b antibody, DNMT3A antibody, Dnmt3a antibody, dnmt3a antibody, dnmt3ab antibody

Background: CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. Dnmt3a is a DNA methyltransferase that is thought to function in de novo methylation, rather than maintenance methylation. The protein localizes to the cytoplasm and nucleus and its expression is developmentally regulated.

Molecular Weight: 101858

Gene ID: 1788

NCBI Accession: NP_072046, NP_715640, NP_783328, NP_783329

UniProt: Q9Y6K1