Kdm6b antibody (N-Term)

Catalog No. ABIN387862

Product Details anti-Kdm6b Antibody

Target: Kdm6b (Lysine (K)-Specific Demethylase 6B (Kdm6b))

Binding Specificity: AA 1-30, N-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Kdm6b antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Predicted Reactivity: M

Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogen: This JMJD3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human JMJD3.

Clone: RB10077

Isotype: Ig Fraction

Application Details

Application Notes: IF: 1:10~50. WB: 1:1000. IHC-P: 1:10~50

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots.

Expiry Date: 6 months

References for anti-Kdm6b antibody (ABIN387862)

Angrisano, Pero, Brancaccio, Coretti, Florio, Pezone, Calabrò, Falco, Keller, Lembo, Avvedimento, Chiariotti: "Cyclical DNA Methylation and Histone Changes Are Induced by LPS to Activate COX-2 in Human Intestinal Epithelial Cells." in: PLoS ONE, Vol. 11, Issue 6, pp. e0156671, (2017) (PubMed).

Petruk, Mariani, De Dominici, Porazzi, Minieri, Cai, Iacovitti, Flomenberg, Calabretta, Mazo: "Structure of Nascent Chromatin Is Essential for Hematopoietic Lineage Specification." in: Cell reports, Vol. 19, Issue 2, pp. 295-306, (2017) (PubMed).

Liao, Kuo, Lu, Wang, Wu: "Generation of an anti-EpCAM antibody and epigenetic regulation of EpCAM in colorectal cancer." in: International journal of oncology, Vol. 46, Issue 4, pp. 1788-800, (2015) (PubMed).

Hashizume, Andor, Ihara, Lerner, Gan, Chen, Fang, Huang, Tom, Ngo, Solomon, Mueller, Paris, Zhang, Petritsch, Gupta, Waldman, James: "Pharmacologic inhibition of histone demethylation as a therapy for pediatric brainstem glioma." in: Nature medicine, (2014) (PubMed).

Lee, Na, Lee, Na, Cho, Wu, Yune, Kim, Ju: "Molecular mechanism of Jmjd3-mediated interleukin-6 gene regulation in endothelial cells underlying spinal cord injury." in: Journal of neurochemistry, Vol. 122, Issue 2, pp. 272-82, (2013) (PubMed).

Ramadoss, Chen, Wang: "Histone demethylase KDM6B promotes epithelial-mesenchymal transition." in: The Journal of biological chemistry, Vol. 287, Issue 53, pp. 44508-17, (2012) (PubMed).

Balasubramanian, Chew, Eckert: "Sulforaphane suppresses polycomb group protein level via a proteasome-dependent mechanism in skin cancer cells." in: Molecular pharmacology, Vol. 80, Issue 5, pp. 870-8, (2011) (PubMed).

Hyland, McDade, McCloskey, Dickson, Arthur, McCance, Patel: "Evidence for alteration of EZH2, BMI1, and KDM6A and epigenetic reprogramming in human papillomavirus type 16 E6/E7-expressing keratinocytes." in: Journal of virology, Vol. 85, Issue 21, pp. 10999-1006, (2011) (PubMed).

Kim, Yoon, Chuong, Oyolu, Wills, Gupta, Baker: "Chromatin and transcriptional signatures for Nodal signaling during endoderm formation in hESCs." in: Developmental biology, Vol. 357, Issue 2, pp. 492-504, (2011) (PubMed).

Lu, Lu, Liao, Yu, Chung, Kao, Wu: "Epithelial cell adhesion molecule regulation is associated with the maintenance of the undifferentiated phenotype of human embryonic stem cells." in: The Journal of biological chemistry, Vol. 285, Issue 12, pp. 8719-32, (2010) (PubMed).

Dai, Lu, Zhang, Shen: "Jmjd3 activates Mash1 gene in RA-induced neuronal differentiation of P19 cells." in: Journal of cellular biochemistry, Vol. 110, Issue 6, pp. 1457-63, (2010) (PubMed).

Toth, Maglinte, Lee, Lee, Wong, Brulois, Lee, Buckley, Laird, Marquez, Jung: "Epigenetic analysis of KSHV latent and lytic genomes." in: PLoS pathogens, Vol. 6, Issue 7, pp. e1001013, (2010) (PubMed).

Peláez, Kalogeropoulou, Ferraro, Voulgari, Pankotai, Boros, Pintzas: "Oncogenic RAS alters the global and gene-specific histone modification pattern during epithelial-mesenchymal transition in colorectal carcinoma cells." in: The international journal of biochemistry & cell biology, Vol. 42, Issue 6, pp. 911-20, (2010) (PubMed).

Long, Huang, Yin, Zhao, Zhao, Lu: "Abnormal expression pattern of histone demethylases in CD4(+) T cells of MRL/lpr lupus-like mice." in: Lupus, Vol. 18, Issue 14, pp. 1327-8, (2009) (PubMed).

Ishii, Wen, Corsa, Liu, Coelho, Allen, Carson, Cavassani, Li, Lukacs, Hogaboam, Dou, Kunkel: "Epigenetic regulation of the alternatively activated macrophage phenotype." in: Blood, Vol. 114, Issue 15, pp. 3244-54, (2009) (PubMed).

Target Details for Kdm6b

Target: Kdm6b (Lysine (K)-Specific Demethylase 6B (Kdm6b))

Alternative Name: JMJD3 (Kdm6b Products)

Synonyms: jmjd3 antibody, kdm6b antibody, si:ch211-237l4.7 antibody, 1700064E03Rik antibody, BC038313 antibody, Jmjd3 antibody, mKIAA0346 antibody, JMJD3 antibody, lysine (K)-specific demethylase 6B antibody, lysine demethylase 6B antibody, lysine (K)-specific demethylase 6B, b antibody, KDM1 lysine (K)-specific demethylase 6B antibody, kdm6b antibody, KDM6B antibody, kdm6bb antibody, Kdm6b antibody

Background: Covalent modification of histones plays critical role in regulating chromatin structure and transcription. While most covalent histone modifications are reversible, only recently has it been established that methyl groups are subject to enzymatic removal from histones. A family of novel JmjC domain-containing histone demethylation (JHDM) enzymes have been identified that perform this specific function. Histone demethylation by JHDM proteins requires cofactors Fe(II) and alpha-ketoglutarate. Family members include JHDM1 (demethylating histone 3 at lysine 36), and JHDM2A as well as JMJD2CH3K9 (both of which demethylate histone 3 at lysine 9). Contributions of histone demethylase activity to tumor development, decreases in cell proliferation, and hormone-dependent transcriptional activation have been observed.

Molecular Weight: 176632

Gene ID: 23135

NCBI Accession: NP_001073893

UniProt: O15054

Pathways: Warburg Effect