DYKDDDDK Tag antibody

Catalog No. ABIN387849

Product Details anti-DYKDDDDK Tag Antibody

Target: DYKDDDDK Tag

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DYKDDDDK Tag antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: DYKDDDDK

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: KLH conjugated synthetic peptide (CDYKDDDDKDYKDDDDK) was used as antigen.

Clone: RB0493

Isotype: IgG

Application Details

Application Notes: WB: 1:2000. WB: 1:2000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

References for anti-DYKDDDDK Tag antibody (ABIN387849)

Xiao, Gao, Hou, Xu, Chang, Wang, Hu, He, Luo, Wang, Peng, Tang, Zhu, Xiong: "Chromatin-remodelling factor Brg1 regulates myocardial proliferation and regeneration in zebrafish." in: Nature communications, Vol. 7, pp. 13787, (2018) (PubMed).

Al-Sady, Greenstein, El-Samad, Braun, Madhani: "Sensitive and Quantitative Three-Color Protein Imaging in Fission Yeast Using Spectrally Diverse, Recoded Fluorescent Proteins with Experimentally-Characterized In Vivo Maturation Kinetics." in: PLoS ONE, Vol. 11, Issue 8, pp. e0159292, (2017) (PubMed).

Target Details for DYKDDDDK Tag

Target: DYKDDDDK Tag

Abstract: DYKDDDDK Tag Products

Target Type: Tag

Background: Epitope tags are useful for the labeling and detection of recombinant proteins using western blotting, immunoprecipitation and immunostaining techniques. The eight amino acid DYKDDDDK peptide is an established and multi-functional epitope tag and can be expressed and detected with a recombinant protein as an amino-terminal or carboxy-terminal fusion (1). Abgent's DYKDDDDK antibody binds to the same epitope recognized by Sigma's Anti-FLAG antibodies. (FLAG is a registered trademark of Sigma-Aldrich Co., which is not affiliated with Abgent).