HA-Tag antibody

Catalog No. ABIN387848

Product Details anti-HA-Tag Antibody

Target: HA-Tag

Reactivity: Please inquire

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HA-Tag antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Chromatin Immunoprecipitation (ChIP)

Sequence: YPYDVPDYA

Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogen: KLH conjugated synthetic peptide encoding HA tag (CYPYDVPDYAYPYDVPDYA) was used as antigen.

Clone: RB0411

Isotype: Ig Fraction

Application Details

Application Notes: IF: 1:10~50. CHIP: 1:100. WB: 1:1000. WB: 1:1000. WB: 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

References for anti-HA-Tag antibody (ABIN387848)

Milewska, Kindler, Vkovski, Zeglen, Ochman, Thiel, Rajfur, Pyrc: "APOBEC3-mediated restriction of RNA virus replication." in: Scientific reports, Vol. 8, Issue 1, pp. 5960, (2019) (PubMed).

Wu, Sun, Ma, Zhu, Zhang, Pan, Li, Liu, Diao, Li: "A C-terminally truncated mouse Best3 splice variant targets and alters the ion balance in lysosome-endosome hybrids and the endoplasmic reticulum." in: Scientific reports, Vol. 6, pp. 27332, (2018) (PubMed).

Baruah, Hitzman, Zhang, Chaudhuri, Mastej, Wary: "The allosteric glycogen synthase kinase-3 inhibitor NP12 limits myocardial remodeling and promotes angiogenesis in an acute myocardial infarction model." in: The Journal of biological chemistry, Vol. 292, Issue 50, pp. 20785-20798, (2018) (PubMed).

Simkin, Robin, Giuliano, Vukolic, Moceri, Guy, Wagner, Lacampagne, Allard, Bendahhou: "Andersen's syndrome mutants produce a knockdown of inwardly rectifying K+ channel in mouse skeletal muscle in vivo." in: Cell and tissue research, Vol. 371, Issue 2, pp. 309-323, (2017) (PubMed).

Di, Wang, Han, Fu, Duerfeldt, Blagg, Mu: "Grp94 Protein Delivers γ-Aminobutyric Acid Type A (GABAA) Receptors to Hrd1 Protein-mediated Endoplasmic Reticulum-associated Degradation." in: The Journal of biological chemistry, Vol. 291, Issue 18, pp. 9526-39, (2016) (PubMed).

Koo, Yue, Deng, Khuri, Sun: "mTOR Complex 2 Stabilizes Mcl-1 Protein by Suppressing Its Glycogen Synthase Kinase 3-Dependent and SCF-FBXW7-Mediated Degradation." in: Molecular and cellular biology, Vol. 35, Issue 13, pp. 2344-55, (2015) (PubMed).

Han, Guan, Wang, Hatzoglou, Mu: "L-type Calcium Channel Blockers Enhance Trafficking and Function of Epilepsy-associated ?1(D219N) Subunits of GABAA Receptors." in: ACS chemical biology, (2015) (PubMed).

Mertz, Tan, Pagala, Bai, Chen, Li, Cho, Shaw, Wang, Peng: "Sequential Elution Interactome Analysis of the Mind Bomb 1 Ubiquitin Ligase Reveals a Novel Role in Dendritic Spine Outgrowth." in: Molecular & cellular proteomics : MCP, Vol. 14, Issue 7, pp. 1898-910, (2015) (PubMed).

Mizutani, Inoue, Omori, Ito, Tamiya-Koizumi, Takagi, Kojima, Nakamura, Iwaki, Nakatochi, Suzuki, Nozawa, Murate: "Increased acid ceramidase expression depends on upregulation of androgen-dependent deubiquitinases, USP2, in a human prostate cancer cell line, LNCaP." in: Journal of biochemistry, (2015) (PubMed).

Oh, Yue, Wang, Tong, Chen, Khuri, Sun: "Suppression of death receptor 5 enhances cancer cell invasion and metastasis through activation of caspase-8/TRAF2-mediated signaling." in: Oncotarget, (2015) (PubMed).

Li, An, Li, Ma, Feng, Zhang, Guo: "DELLA Proteins Interact with FLC to Repress the Flowering Transition." in: Journal of integrative plant biology, (2015) (PubMed).

Zhao, Yue, Khuri, Sun: "mTOR complex 2 is involved in regulation of Cbl-dependent c-FLIP degradation and sensitivity of TRAIL-induced apoptosis." in: Cancer research, Vol. 73, Issue 6, pp. 1946-57, (2013) (PubMed).

Oh, Yue, Zhou, Balko, Black, Owonikoko, Khuri, Sun: "Oncogenic Ras and B-Raf proteins positively regulate death receptor 5 expression through co-activation of ERK and JNK signaling." in: The Journal of biological chemistry, Vol. 287, Issue 1, pp. 257-67, (2012) (PubMed).

Li, Ren, Yue, Chen, Khuri, Sun: "The novel Akt inhibitor API-1 induces c-FLIP degradation and synergizes with TRAIL to augment apoptosis independent of Akt inhibition." in: Cancer prevention research (Philadelphia, Pa.), Vol. 5, Issue 4, pp. 612-20, (2012) (PubMed).

Zhao, Yue, Lonial, Khuri, Sun: "The NEDD8-activating enzyme inhibitor, MLN4924, cooperates with TRAIL to augment apoptosis through facilitating c-FLIP degradation in head and neck cancer cells." in: Molecular cancer therapeutics, Vol. 10, Issue 12, pp. 2415-25, (2011) (PubMed).

Fan, Li, Yue, Khuri, Sun: "The eIF4E/eIF4G interaction inhibitor 4EGI-1 augments TRAIL-mediated apoptosis through c-FLIP Down-regulation and DR5 induction independent of inhibition of cap-dependent protein translation." in: Neoplasia (New York, N.Y.), Vol. 12, Issue 4, pp. 346-56, (2010) (PubMed).

Guo, Wang, Wang, Huang, Liang, Chen: "Dissection of the relationship between RACK1 and heterotrimeric G-proteins in Arabidopsis." in: Plant & cell physiology, Vol. 50, Issue 9, pp. 1681-94, (2009) (PubMed).

Scarselli, Donaldson: "Constitutive internalization of G protein-coupled receptors and G proteins via clathrin-independent endocytosis." in: The Journal of biological chemistry, Vol. 284, Issue 6, pp. 3577-85, (2009) (PubMed).

Lin, Liu, Yue, Benbrook, Berlin, Khuri, Sun: "Involvement of c-FLIP and survivin down-regulation in flexible heteroarotinoid-induced apoptosis and enhancement of TRAIL-initiated apoptosis in lung cancer cells." in: Molecular cancer therapeutics, Vol. 7, Issue 11, pp. 3556-65, (2008) (PubMed).

Wang, Kwak, Zeng, Ellis, Chen, Schiefelbein, Chen: "TRICHOMELESS1 regulates trichome patterning by suppressing GLABRA1 in Arabidopsis." in: Development (Cambridge, England), Vol. 134, Issue 21, pp. 3873-82, (2007) (PubMed).

Target Details for HA-Tag

Target: HA-Tag

Abstract: HA-Tag Products

Target Type: Tag

Background: Epitope tags consisting of short sequences recognized by well-characterizated antibodies have been widely used in the study of protein expression in various systems. The HA tag (YPYDVPDYAYPYDVPDYA) is an established example. Abgent's anti-HA polyclonal antibody provides a simple solution to detect the expression of an HA-tagged protein in cells.