His Tag antibody

Catalog No. ABIN387750

Product Details anti-His Tag Antibody

Target: His Tag

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This His Tag antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: HHHHHH

Purification: This antibody is purified through a protein G column, followed by dialysis against PBS.

Immunogen: Purified recombinant HIS-tagged fusion protein and poly-HIS peptide were used to produced this monoclonal antibody.

Clone: 6AT18

Isotype: IgG1

Application Details

Application Notes: WB: 1:8000. WB: 1:1,000. WB: 1:2000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified monoclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots.

Expiry Date: 6 months

References for anti-His Tag antibody (ABIN387750)

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Sun, Ji, Liang, Du, Jiang, Wang, Kai, Zhang, Zhai, Chen, Wang, Leng: "Suppressing ABA uridine diphosphate glucosyltransferase (SlUGT75C1) alters fruit ripening and the stress response in tomato." in: The Plant journal : for cell and molecular biology, Vol. 91, Issue 4, pp. 574-589, (2018) (PubMed).

Wang, Gu, Zhuang, Zhong, Yang, Wang: "Screening and Molecular Evolution of a Single Chain Variable Fragment Antibody (scFv) against Citreoviridin Toxin." in: Journal of agricultural and food chemistry, Vol. 64, Issue 40, pp. 7640-7648, (2017) (PubMed).

Wang, Zhao: "A simple strategy for extracellular production of CotA laccase in Escherichia coli and decolorization of simulated textile effluent by recombinant laccase." in: Applied microbiology and biotechnology, Vol. 101, Issue 2, pp. 685-696, (2017) (PubMed).

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Rath, Hilton, Sisco, Van Horn: "Implications of Human Transient Receptor Potential Melastatin 8 (TRPM8) Channel Gating from Menthol Binding Studies of the Sensing Domain." in: Biochemistry, Vol. 55, Issue 1, pp. 114-24, (2016) (PubMed).

Tian, Qu, Bao, Gao, Liu, Wang, Sun, Ding, Yu: "Construction of pTM series plasmids for gene expression in Brucella species." in: Journal of microbiological methods, Vol. 123, pp. 18-23, (2016) (PubMed).

Militi, Maywood, Sandate, Chesham, Barnard, Parsons, Vibert, Joynson, Partch, Hastings, Nolan: "Early doors (Edo) mutant mouse reveals the importance of period 2 (PER2) PAS domain structure for circadian pacemaking." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 113, Issue 10, pp. 2756-61, (2016) (PubMed).

Brown, Nicholson, Arama, Mercer, Thomson, Jovanovic: "γ-Aminobutyric Acid Type A (GABAA) Receptor Subunits Play a Direct Structural Role in Synaptic Contact Formation via Their N-terminal Extracellular Domains." in: The Journal of biological chemistry, Vol. 291, Issue 27, pp. 13926-42, (2016) (PubMed).

Kim, Pai, Sato, Person, Nakamura, Macdonald: "Region-specific activation of oskar mRNA translation by inhibition of Bruno-mediated repression." in: PLoS genetics, Vol. 11, Issue 2, pp. e1004992, (2015) (PubMed).

Grigoroudis, McInnes, Premnath, Kontopidis: "Efficient soluble expression of active recombinant human cyclin A2 mediated by E. coli molecular chaperones." in: Protein expression and purification, Vol. 113, pp. 8-16, (2015) (PubMed).

Yarosh, Tapescu, Thompson, Qiu, Mallory, Fu, Lynch: "TRAP150 interacts with the RNA-binding domain of PSF and antagonizes splicing of numerous PSF-target genes in T cells." in: Nucleic acids research, Vol. 43, Issue 18, pp. 9006-16, (2015) (PubMed).

Lv, Lei, Hong, Wang, Zhang, Zhou, Sun, Ma, Shen, Zhu: "Venom allergen 5 is Associated With Deltamethrin Resistance in Culex pipiens pallens (Diptera: Culicidae)." in: Journal of medical entomology, Vol. 52, Issue 4, pp. 672-82, (2015) (PubMed).

Bhandari, Lopez-Sanchez, To, Lo, Aznar, Leyme, Gupta, Niesman, Maddox, Garcia-Marcos, Farquhar, Ghosh: "Cyclin-dependent kinase 5 activates guanine nucleotide exchange factor GIV/Girdin to orchestrate migration-proliferation dichotomy." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 112, Issue 35, pp. E4874-83, (2015) (PubMed).

Shi, Zhang, Guo, Zhang, Hu, Xi, Miao, Li: "131I therapy mediated by sodium/iodide symporter combined with kringle 5 has a synergistic therapeutic effect on glioma." in: Oncology reports, (2015) (PubMed).

Liu, Novick: "Bem1p contributes to secretory pathway polarization through a direct interaction with Exo70p." in: The Journal of cell biology, Vol. 207, Issue 1, pp. 59-72, (2014) (PubMed).

Hufnagel, DePas, Chapman: "The disulfide bonding system suppresses CsgD-independent cellulose production in Escherichia coli." in: Journal of bacteriology, Vol. 196, Issue 21, pp. 3690-9, (2014) (PubMed).

Faure, Nagarajan, Hwang, Montgomery, Khan, John, Koulen, Blancaflor, Chapman: "Synthesis of phenoxyacyl-ethanolamides and their effects on fatty acid amide hydrolase activity." in: The Journal of biological chemistry, Vol. 289, Issue 13, pp. 9340-51, (2014) (PubMed).

Malmirchegini, Sjodt, Shnitkind, Sawaya, Rosinski, Newton, Klebba, Clubb: "Novel mechanism of hemin capture by Hbp2, the hemoglobin-binding hemophore from Listeria monocytogenes." in: The Journal of biological chemistry, Vol. 289, Issue 50, pp. 34886-99, (2014) (PubMed).

Wang, Xiang, Zhang, Chen, Zhong, Wang: "Development of a functional antibody by using a green fluorescent protein frame as the template." in: Applied and environmental microbiology, Vol. 80, Issue 14, pp. 4126-37, (2014) (PubMed).

Target Details for His Tag

Target: His Tag

Abstract: His Tag Products

Target Type: Tag

Background: Epitope tags consisting of short sequences recognized by well-characterized monoclonal antibodies have been widely used in the study of protein expression in various systems. The 6xHIS tag (HHHHHH), recognized by the monoclonal antibody clone 6AT18 provides an established example of this application. 6xHIS-tagged fusion proteins are easily purified from cell lysates by affinity chromatography using Nickel-Sepharose resin. Abgent's anti-6xHIS monoclonal antibody provides a simple solution to detect the expression of HIS-tagged fusion proteins in cells.

Gene ID: 12406