Western Blotting (WB), ELISA, Immunofluorescence (IF)
Specificity
THETM c-Myc Antibody [HRP], mAb, Mouse recognizes C-terminal, N-terminal, and internal c-Myc-tagged fusion proteins expressed in prokaryotic or eukaryotic cells. It also recognizes the c-Myc gene in human cancers, such as Hela whole cell lysate.
Characteristics
Mouse Anti-c-Myc [HRP] Monoclonal Antibody is suitable for detecting the expression level of c-Myc in fusion proteins.
Purification
Protein A affinity column
Immunogen
A synthetic peptide corresponding - AA 410-419 (EQKLISEEDL) coupled - KLH
MYC
Reactivity: Human
WB, ELISA
Host: Mouse
Monoclonal
7-00E-10
unconjugated
Application Notes
Working concentrations for specific applications should be determined by the investigator. Appropriate concentrations will be affected by severalfactors, including secondary antibody affinity, antigen concentration,sensitivity of detection method, temperature, and length of incubations,etc. The suitability of this antibody for applications other than thoselisted below has not been determined. The following concentration rangesare recommended starting points for this product. ELISA: 0.05- 0.2 µg/mL Western blot: 0.1-1 µg/mL Immunoprecipitation (IP): 1 µg/mL Immunofluorescent staining: 1 µg/mL Other applications: user-optimized
This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Avoid repeated freezing and thawing cycles.
Storage
4 °C/-20 °C
Storage Comment
The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below.