Applications: FC - Quality tested , IHC-FS - Reported in literature , ICC - Reported in literature , IP - Reported in literature , Block - Reported in literature
Working Dilutions: Flow Cytometry Purified (UNLB) antibody 1 g/106 cells FITC and PE conjugates 10 L/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L
Sample Volume
1 mL
Restrictions
For Research Use only
Concentration
0.1 mg/mL
Buffer
0.1 mg of purified immunoglobulin in 1.0 mL of borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added
Preservative
Without preservative
Handling Advice
Each reagent is stable for the period shown on the bottle label if stored as directed.
CD49b, a member of the integrin family of extracellular matrix and cell adhesion molecules, is the integrin α 2 chain. CD49b associates noncovalently with CD29, the integrin β 1 subunit, to form the heterodimer integrin a2b1 that binds laminin and collagen. It is expressed on B and T lymphocytes, NK cells, monocytes, platelets, fibroblasts and long-term cultured T cells. CD49b/CD29 mediates the Mg ++ - dependent adhesion of platelets to collagen. It may also be utilized by fibroblasts for the reorganization of the collagen matrix during would healing.