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CD32/CD16 antibody (SPRD)

CD32/CD16 Reactivity: Mouse FACS Host: Rat Monoclonal 93 SPRD
Catalog No. ABIN376709
  • Target See all CD32/CD16 products
    CD32/CD16
    Reactivity
    Mouse
    Host
    • 44
    • 1
    • 1
    Rat
    Clonality
    • 41
    • 1
    • 1
    Monoclonal
    Conjugate
    • 13
    • 6
    • 6
    • 4
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This CD32/CD16 antibody is conjugated to SPRD
    Application
    • 45
    • 9
    • 7
    • 7
    • 3
    • 3
    • 1
    • 1
    Flow Cytometry (FACS)
    Brand
    Spectral Red ™
    Specificity
    Mouse CD16/32
    Characteristics
    Rat Anti-Mouse CD16/32-SPRD
    Purification
    Purified
    Immunogen
    VH81X Tg B cells and T220 stromal cell line
    Clone
    93
    Isotype
    IgG2a
  • Application Notes
    • Applications: FC - Quality tested , IHC-FS - Reported in literature , IP - Reported in literature , Block - Reported in literature
    • Working Dilutions: Flow Cytometry Purified (UNLB) antibody 3 g/106 cells FITC and BIOT conjugates 3 g/106 cells PE, APC, SPRD, PE/CY5.5, and PE/CY7 conjugates 0.2 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L
    Comment

    Blocking of g receptors

    Sample Volume
    1 mL
    Restrictions
    For Research Use only
  • Concentration
    0.1 mg/mL
    Buffer
    0.1 mg in 1.0 mL of PBS/Sodium azide and a stabilizing agent
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Do not freeze!
    Protect conjugated products from light.

    Each reagent is stable for the period shown on the bottle label if stored as directed.
    Storage
    4 °C
    Storage Comment
    Store at 2-8°C
  • Target
    CD32/CD16
    Alternative Name
    CD16/32 (CD32/CD16 Products)
    Background
    The lymphocyte Fc γ receptors recognize the Fc portion of IgG, presented either as immune complexes or as free antibody. The different classes of receptors are distinct because of varying size, tissue distribution and affinity for IgGs. The Fc type II receptor is expressed on a wide variety of cells including B cells, hematopoietic cells, monocyte/macrophages, neutrophils, platelets, Langerhans cells, eosinophils, basophils, trophoblasts, and endothelial cells of the placenta. The Fc γ type III receptors are higher affinity than the type II and are expressed on macrophages, NK cells and neutrophils. Both types of receptors can be expressed on the same cell and in varying ratios. The receptors are constitutively expressed, although cytokines and lymphokines can modulate their expression. Besides identifying Fc γ R + cells, monoclonal antibodies to the Fc γ II/III receptor have been used to block Fc receptor binding of IgG, Fc- mediated signal transduction and effector functions, clearance of immune complexes and to attenuate infection by organisms dependent on Fc γ R for parasitic invasion.
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