Goat anti-Human IgG Antibody (PE)

Catalog No. ABIN376510

Product Details

Target: IgG

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: PE

Application: ELISA, Flow Cytometry (FACS)

Specificity: Reacts with the heavy chain of human IgG

Characteristics: Goat Anti-Human IgG-PE

Purification: Purification Method: Affinity chromatography on human IgG covalently linked to agarose.

Isotype: IgG

Application Details

Application Notes:

• Applications: Quality tested applications include - ELISA , FLISA , FC ,
• Other referenced applications include - ELISPOT , IHC-FS , IHC-PS , ICC , WB , IP , Multiplex , Depletion
• Working Dilutions: ELISA AP conjugate 1:2,000 - 1:4,000 HRP conjugate 1:4,000 - 1:8,000 BGAL conjugate 1:500 BIOT conjugate 1:5,000 - 1:20,000 FLISA FITC, TRITC, TXRD, AF488, and AF555 conjugates 1:100 - 1:400 PE and AF647 conjugates 1 g/mL Flow Cytometry Purified (UNLB) antibody 1 g/106 cells FITC, AF488, and BIOT conjugates 1 g/106 cells PE and AF647 conjugates 0.1 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: 0.5 mg in 1.0 mL of PBS/Sodium azide and a stabilizing agent

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze!
Protect conjugated products from light.
Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: 4 °C

Storage Comment: Store at 2-8°C

References

Gilchuk, Kuzmina, Ilinykh, Huang, Gunn, Bryan, Davidson, Doranz, Turner, Fusco, Bramble, Hoff, Binshtein, Kose, Flyak, Flinko, Orlandi, Carnahan, Parrish, Sevy, Bombardi, Singh, Mukadi et al.: "Multifunctional Pan-ebolavirus Antibody Recognizes a Site of Broad Vulnerability on the Ebolavirus Glycoprotein. ..." in: Immunity, Vol. 49, Issue 2, pp. 363-374.e10, (2019) (PubMed).

Zhang, Gorman, Geng, Liu, Lin, Tsybovsky, Go, Dey, Andine, Kwon, Patel, Gururani, Uddin, Guzzo, Cimbro, Miao, McKee, Chuang, Martin, Sironi, Malnati, Desaire, Berger, Mascola, Dolan, Kwong, Lusso: "Interdomain Stabilization Impairs CD4 Binding and Improves Immunogenicity of the HIV-1 Envelope Trimer." in: Cell host & microbe, Vol. 23, Issue 6, pp. 832-844.e6, (2019) (PubMed).

Järvinen, Wang, Seppo, Zand: "Novel multiplex assay for profiling influenza antibodies in breast milk and serum of mother-infant pairs." in: F1000Research, Vol. 7, pp. 1822, (2018) (PubMed).

Kowalczyk-Quintas, Chevalley, Willen, Jandus, Vigolo, Schneider: "Inhibition of Membrane-Bound BAFF by the Anti-BAFF Antibody Belimumab." in: Frontiers in immunology, Vol. 9, pp. 2698, (2018) (PubMed).

Cao, Pauthner, Andrabi, Rantalainen, Berndsen, Diedrich, Menis, Sok, Bastidas, Park, Delahunty, He, Guenaga, Wyatt, Schief, Ward, Yates, Burton, Paulson: "Differential processing of HIV envelope glycans on the virus and soluble recombinant trimer." in: Nature communications, Vol. 9, Issue 1, pp. 3693, (2018) (PubMed).

Target Details

Target: IgG

Abstract: IgG Products

Target Type: Antibody