Mouse anti-Human IgG (Fc Region) Antibody (Cy5)
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- Target See all IgG products
- IgG
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Binding Specificity
- Fc Region
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- Cy5
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Application
- ELISA, Flow Cytometry (FACS)
- Specificity
- Reacts with the Fc portion of the heavy chain of all subclasses of human IgG as demonstrated by ELISA
- Characteristics
- Mouse Anti-Human IgG Fc-CY5
- Purification
- Purified
- Immunogen
- Unknown
- Clone
- H2
- Isotype
- IgG2b
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- Application Notes
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- Applications: ELISA - Quality tested , FLISA - Quality tested FC - Quality tested , ELISPOT - Reported in literature , WB - Reported in literature , Multiplex - Reported in literature
- Working Dilutions: ELISA AP conjugate 1:2,000 - 1:8,000 HRP conjugate 1:4,000 - 1:8,000 BIOT conjugate 1:10,000 - 1:20,000 FLISA FITC conjugate 1:200 - 1:600 AF488 and AF555 conjugates 1:100 - 1:400 PE and AF647 conjugates 1 g/mL Flow Cytometry Purified (UNLB) antibody 1 g/106 cells FITC, BIOT, and AF488 conjugates 1 g/106 cells CY5 conjugate 0.3 g/106 cells PE, APC, SPRD, and AF647 conjugates 0.1 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L
- Comment
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Excitation / Emission wavelength: 652 nm / 672 nm
- Restrictions
- For Research Use only
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- Concentration
- 0.5 mg/mL
- Buffer
- 0.1 mg in 0.2 mL of PBS/Sodium azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
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Protect conjugated products from light.
Each reagent is stable for the period shown on the bottle label if stored as directed. - Storage
- 4 °C
- Storage Comment
- Store at 2-8°C
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- Target
- IgG
- Abstract
- IgG Products
- Target Type
- Antibody
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