CD3 epsilon antibody (Cy5)

Catalog No. ABIN376051

Product Details anti-CD3 epsilon Antibody (Cy5)

Target: CD3 epsilon (CD3E)

Reactivity: Mouse

Host: Hamster

Clonality: Monoclonal

Conjugate: This CD3 epsilon antibody is conjugated to Cy5

Application: Flow Cytometry (FACS)

Specificity: Mouse CD3 Mr 25 kDa

Characteristics: Hamster Anti-Mouse CD3e-CY5

Purification: Purified

Immunogen: Immune complexes isolated from C6VL-BS thymoma cells

Clone: 500-A2

Isotype: IgG

Application Details

Application Notes:

• Applications: FC - Quality tested , IHC-FS - Reported in literature , IP - Reported in literature , Activ - Reported in literature
• Working Dilutions: Flow Cytometry FITC conjugate 3 g/106 cells BIOT conjugate 1 g/106 cells PE, APC, and APC/CY5.5 conjugates 0.3 g/106 cells SPRD and CY5 conjugates 0.1 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 l

Comment:

In vitro activation of T cells

Sample Volume: 1 mL

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: 0.1 mg in 1.0 mL of PBS/Sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect conjugated products from light.
Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: 4 °C

Storage Comment: Store at 2-8°C

Target Details for CD3 epsilon

Target: CD3 epsilon (CD3E)

Alternative Name: CD3e (CD3E Products)

Background: CD3 ε, a member of the immunoglobulin superfamily of cell surface receptors, is comprised of five invariable chains ranging in size from 16-28 kDa and is closely associated with the T cell antigen receptor (TCR). CD3 ε is expressed on all T cells of all mouse strains. CD3 plays a major role in signaling during antigen recognition, leading to T-cell activation.

Pathways: TCR Signaling, CXCR4-mediated Signaling Events, Ubiquitin Proteasome Pathway