CD32/CD16 antibody (Biotin)

Catalog No. ABIN375939

Product Details anti-CD32/CD16 Antibody (Biotin)

Target: CD32/CD16

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This CD32/CD16 antibody is conjugated to Biotin

Application: Flow Cytometry (FACS)

Specificity: Mouse CD16/32

Characteristics: Rat Anti-Mouse CD16/32-BIOT

Purification: Affinity chromatography

Immunogen: VH81X Tg B cells and T220 stromal cell line

Clone: 93

Isotype: IgG2a

Application Details

Application Notes:

• Applications: FC - Quality tested , IHC-FS - Reported in literature , IP - Reported in literature , Block - Reported in literature
• Working Dilutions: Flow Cytometry Purified (UNLB) antibody 3 g/106 cells FITC and BIOT conjugates 3 g/106 cells PE, APC, SPRD, PE/CY5.5, and PE/CY7 conjugates 0.2 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L

Comment:

Blocking of g receptors

Sample Volume: 1 mL

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: 0.5 mg in 1.0 mL of PBS/Sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect conjugated products from light.
Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: 4 °C

Storage Comment: Store at 2-8°C

Target Details for CD32/CD16

Target: CD32/CD16

Alternative Name: CD16/32 (CD32/CD16 Products)

Background: The lymphocyte Fc γ receptors recognize the Fc portion of IgG, presented either as immune complexes or as free antibody. The different classes of receptors are distinct because of varying size, tissue distribution and affinity for IgGs. The Fc type II receptor is expressed on a wide variety of cells including B cells, hematopoietic cells, monocyte/macrophages, neutrophils, platelets, Langerhans cells, eosinophils, basophils, trophoblasts, and endothelial cells of the placenta. The Fc γ type III receptors are higher affinity than the type II and are expressed on macrophages, NK cells and neutrophils. Both types of receptors can be expressed on the same cell and in varying ratios. The receptors are constitutively expressed, although cytokines and lymphokines can modulate their expression. Besides identifying Fc γ R + cells, monoclonal antibodies to the Fc γ II/III receptor have been used to block Fc receptor binding of IgG, Fc- mediated signal transduction and effector functions, clearance of immune complexes and to attenuate infection by organisms dependent on Fc γ R for parasitic invasion.