Sca-1/Ly-6A/E antibody (SPRD)
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- Target See all Sca-1/Ly-6A/E (Ly6a) Antibodies
- Sca-1/Ly-6A/E (Ly6a) (Lymphocyte Antigen 6 Complex, Locus A (Ly6a))
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Reactivity
- Mouse
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Host
- Rat
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Clonality
- Monoclonal
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Conjugate
- This Sca-1/Ly-6A/E antibody is conjugated to SPRD
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Application
- Flow Cytometry (FACS)
- Brand
- Spectral Red ™
- Specificity
- Mouse Ly-6A/E (Ly-6A.2 & Ly-6E.1), Mr 18 kDa
- Characteristics
- Rat Anti-Mouse Ly-6A/E-SPRD
- Purification
- Purified
- Immunogen
- IL-2 dependent mouse (C57BL/6) cytotoxic T cell line CTL-L
- Clone
- D7
- Isotype
- IgG2a
- Top Product
- Discover our top product Ly6a Primary Antibody
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- Application Notes
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- Applications: FC - Quality tested , IHC-FS - Reported in literature , IHC-PS - Reported in literature , IP - Reported in literature , WB - Reported in literature , Costim - Reported in literature
- Working Dilutions: Flow Cytometry FITC, BIOT, and AF488 conjugates 1 g/106 cells PE, APC, SPRD, PE/CY5.5, PACBLU, and AF647 conjugates 0.2 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L
- Comment
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In vitro lymphocyte activation
- Sample Volume
- 1 mL
- Restrictions
- For Research Use only
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- Concentration
- 0.1 mg/mL
- Buffer
- 0.1 mg in 1.0 mL of PBS/Sodium azide and a stabilizing agent
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
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Do not freeze!
Protect conjugated products from light.
Each reagent is stable for the period shown on the bottle label if stored as directed. - Storage
- 4 °C
- Storage Comment
- Store at 2-8°C
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- Target
- Sca-1/Ly-6A/E (Ly6a) (Lymphocyte Antigen 6 Complex, Locus A (Ly6a))
- Alternative Name
- Ly-6A/E (Ly6a Products)
- Synonyms
- Ly-6A.2 antibody, Ly-6A/E antibody, Ly-6E.1 antibody, Sca-1 antibody, Sca1 antibody, TAP antibody, lymphocyte antigen 6 complex, locus A antibody, Ly6a antibody
- Background
- Ly-6A/E, also known as Sca-1, is a member of the Ly-6 multigene family of type V glycophosphatidylinositol-anchored cell surface proteins. It is expressed on multipotent hematopoietic stem cells in bone marrow of mice with both the Ly-6.1 and Ly-6.2 allotypes. In the periphery, Ly-6A/E exhibits a pattern of expression which is based on differences between the two allotypes. Ly-6.1 strains (e.g., A, BALB/c, CBA, C3H/He, DBA/1, NZB) have few Ly-6A/E + resting peripheral lymphocytes, whereas Ly-6.2 strains (e.g., AKR, C57BL, C57BR, C57L, DBA/2, PL, SJL, SWR, 129) have relatively high numbers of Ly-6A/E + lymphocytes. The expression of Ly-6A/E is dramatically upregulated in all strains upon cellular activation.
- Pathways
- Sensory Perception of Sound, Activated T Cell Proliferation
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