Applications: FC - Quality tested , IHC-FS - Reported in literature , IP - Reported in literature , Adhesion - Reported in literature , Block - Reported in literature
Working Dilutions: Flow Cytometry FITC and BIOT conjugates 2 g/106 cells PE conjugates 0.2 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L
Sample Volume
2 mL
Restrictions
For Research Use only
Concentration
0.1 mg/mL
Buffer
0.1 mg in 1.0 mL or 0.2 mg in 2.0 mL of PBS/Sodium azide and a stabilizing agent
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Do not freeze! Protect conjugated products from light. Each reagent is stable for the period shown on the bottle label if stored as directed.
Storage
4 °C
Storage Comment
Store at 2-8°C
Fistonich, Zehentmeier, Bednarski, Miao, Schjerven, Sleckman, Pereira: "Cell circuits between B cell progenitors and IL-7+ mesenchymal progenitor cells control B cell development." in: The Journal of experimental medicine, Vol. 215, Issue 10, pp. 2586-2599, (2018) (PubMed).
VLA-4 belongs to the β 1 -integrin family, which functions both as cell-matrix and cell-cell receptors. As a matrix receptor VLA-4 binds to an alternatively spliced domain of fibronectin, while as a cellular receptor it associates with its counter-receptor ligand VCAM-1. In addition to CD44/pgp-1, VLA-4 has been shown to be important for lymphopoiesis, and is also believed to be important for extravasation of lymphocytes into sites of inflammation as a second signal in addition to LFA/ICAM-1. CD49d/VLA-4 is expressed in low amounts on resting lymphocytes and monocytes, is absent or minimally detectable on stromal cells, whereas virtually all lymphoid and myeloid cells in bone marrow and in long-term culture are positive.