Sca-1/Ly-6A/E antibody (APC)

Catalog No. ABIN375409

Product Details anti-Sca-1/Ly-6A/E Antibody (APC)

Target: Sca-1/Ly-6A/E (Ly6a) (Lymphocyte Antigen 6 Complex, Locus A (Ly6a))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This Sca-1/Ly-6A/E antibody is conjugated to APC

Application: Flow Cytometry (FACS)

Specificity: Mouse Ly-6A/E (Ly-6A.2 & Ly-6E.1), Mr 18 kDa

Characteristics: Rat Anti-Mouse Ly-6A/E-APC

Purification: Purified

Immunogen: IL-2 dependent mouse (C57BL/6) cytotoxic T cell line CTL-L

Clone: D7

Isotype: IgG2a

Application Details

Application Notes:

• Applications: FC - Quality tested , IHC-FS - Reported in literature , IHC-PS - Reported in literature , IP - Reported in literature , WB - Reported in literature , Costim - Reported in literature
• Working Dilutions: Flow Cytometry FITC, BIOT, and AF488 conjugates 1 g/106 cells PE, APC, SPRD, PE/CY5.5, PACBLU, and AF647 conjugates 0.2 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L

Comment:

In vitro lymphocyte activation

Sample Volume: 1 mL

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: 0.1 mg in 1.0 mL of PBS/Sodium azide and a stabilizing agent

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze!
Protect conjugated products from light.
Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: 4 °C

Storage Comment: Store at 2-8°C

References for anti-Sca-1/Ly-6A/E antibody (APC) (ABIN375409)

Filip, Mokrý, Vávrová, Sinkorová, Mičuda, Sponer, Filipová, Hrebíková, Dayanithi: "The peripheral chimerism of bone marrow-derived stem cells after transplantation: regeneration of gastrointestinal tissues in lethally irradiated mice." in: Journal of cellular and molecular medicine, (2014) (PubMed).

Vávrová, Sinkorová, Rezáčová, Tichý, Filip, Mokrý, Lukášová: "Irradiated stem cells and ageing of the haematopoietic system." in: Radiation and environmental biophysics, (2012) (PubMed).

Target Details for Sca-1/Ly-6A/E

Target: Sca-1/Ly-6A/E (Ly6a) (Lymphocyte Antigen 6 Complex, Locus A (Ly6a))

Alternative Name: Ly-6A/E (Ly6a Products)

Synonyms: Ly-6A.2 antibody, Ly-6A/E antibody, Ly-6E.1 antibody, Sca-1 antibody, Sca1 antibody, TAP antibody, lymphocyte antigen 6 complex, locus A antibody, Ly6a antibody

Background: Ly-6A/E, also known as Sca-1, is a member of the Ly-6 multigene family of type V glycophosphatidylinositol-anchored cell surface proteins. It is expressed on multipotent hematopoietic stem cells in bone marrow of mice with both the Ly-6.1 and Ly-6.2 allotypes. In the periphery, Ly-6A/E exhibits a pattern of expression which is based on differences between the two allotypes. Ly-6.1 strains (e.g., A, BALB/c, CBA, C3H/He, DBA/1, NZB) have few Ly-6A/E + resting peripheral lymphocytes, whereas Ly-6.2 strains (e.g., AKR, C57BL, C57BR, C57L, DBA/2, PL, SJL, SWR, 129) have relatively high numbers of Ly-6A/E + lymphocytes. The expression of Ly-6A/E is dramatically upregulated in all strains upon cellular activation.

Pathways: Sensory Perception of Sound, Activated T Cell Proliferation