HNF1B antibody (Internal Region)

Catalog No. ABIN375249

Product Details anti-HNF1B Antibody

Target: HNF1B (HNF1 Homeobox B (HNF1B))

Binding Specificity: Internal Region

Reactivity: Human, Mouse, Rat, Zebrafish (Danio rerio), Dog, Cow, Pig

Host: Goat

Clonality: Polyclonal

Conjugate: This HNF1B antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA)

Sequence: C-QAYDRQKNPS KEER

Specificity: This antibody is expected to recognize both reported isoforms (NP_000449.1 and NP_001159395.1).

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat, Canine, Pig, Cow, Zebrafish.
Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide

Immunogen: Peptide with sequence from the internal region of the protein according to NP_000449.1, NP_001159395.1. Genename: HNF1B

Application Details

Application Notes: Peptide ELISA: 1/64000. Western Blot: 0.1 - 0.3 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for HNF1B

Target: HNF1B (HNF1 Homeobox B (HNF1B))

Alternative Name: HNF1 beta / TCF2 (HNF1B Products)

Background: TCF2 encodes transcription factor 2, a liver specific factor of the homeobox containing basic helix turn helix family. The TCF2 protein is believed to form heterodimers with another liver specific member of this transcription factor family, TCF1, depending on the TCF2 isoform, the result may be to activate or inhibit transcription of target genes. A mutation of TCF2 that disrupts normal function has been identified as the cause of MODY5 (Maturity Onset of Diabetes, Type 5). A third human transcript variant is believed to exist based on such a variant in the rat. However, to date such an mRNA species has not been isolated. TCF2 probably binds to the inverted palindrome 5' GTTAATNATTAAC 3'.Synonyms: HNF-1-beta, HNF-1B, Hepatocyte nuclear factor 1-beta, LFB3, TCF-2, Transcription factor 2, Variant hepatic nuclear factor 1, vHNF1

Gene ID: 6928

NCBI Accession: NP_000449

UniProt: P35680

Pathways: Hormone Transport, Stem Cell Maintenance, Tube Formation