Peptide ELISA: Detection Limit: 1/64000. Western blot: 0.03-0.1 μg/mL. Approx 55 kDa band observed in lysates of cell line HepG2(calculated MW of 50.5 kDa according to NP_000390.2). Immunohistochemistry: 3-5 μg/mL. In paraffin embedded Human Colon shows nuclearstaining of absorptive cells. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
0.5 mg/mL
Buffer
Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Avoid repeated freezing and thawing.
Storage
4 °C/-20 °C
Storage Comment
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
ERG2 is a sequence-specific transcription factor, that binds to two specific DNA sites located in the promoter region of HOXA4. Defects in EGR2 are a cause of congenital hypomyelination neuropathy (CHN), which can be inherited in either an autosomal dominant or recessive manner. Recessive CHN can present as Charcot-Marie-Tooth disease type 4E, type 1D, or Dejerine-Sottas syndrome (DSS), a group of neuropathies associated with decreased nerve conduction velocities.Synonyms: AT591, EGR-2, Early growth response protein 2, KROX20, Krox 20, Zinc finger protein Krox-20